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The transcriptional factor GATA-4 negatively regulates Hsp70 transcription in Crassostrea hongkongensis

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Abstract

To better explore the application potential of heat shock protein Hsp70s in diverse areas including biomonitoring, a further investigation of the details of the regulatory mechanism governing Hsp70 transcription is required. A transcriptional factor ChGATA-4 that displayed affinity to the ChHsp70 promoter of Crassostrea hongkongensis was isolated and identified by DNA affinity purification as well as mass spectrometry analysis. The ChGATA-4 cDNA is 2162 bp in length and the open reading frame encodes a polypeptide containing 482 amino acids with a conserved zinc finger domain. The over-expression of ChGATA-4 significantly inhibited the expression of ChHsp70 promoter in heterologous HEK293T cells. However, the depletion of ChGATA-4 mRNA by RNAi technique resulted in significant increase of ChHsp70 transcription in oyster hemocytes. The RT-PCR results demonstrated that the transcription of both ChHsp70 and ChGATA-4 were induced by heat, Cd, or NP (Nonyl phenol) stress. This suggested a potential correlation between ChHsp70 and ChGATA-4 in the stress-mediated genetic regulatory cascade. This study demonstrated that ChGATA-4 acts in a negative manner in controlling ChHsp70 transcription in C. hongkongensis and promotes to further understand the mechanisms leading Hsp70 transcription.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 41476100), the Key Natural Science Foundation of Guangdong Province (No. S2013020012720), and Key-Area Research and Development Program of Guangdong Province (2019B020217001), as well as the Shellfish and Large Algae Industry Innovation Team Project of Guangdong Province (2019KJ146).

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  1. Tinglong Hou and Delin Xu have contributed equally to this work.

Authors and Affiliations

  1. Department of Ecology, Institute of Hydrobiology, School of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Jinan University, Guangzhou, 510632, People’s Republic of China

    Tinglong Hou, Delin Xu, Miao Cui, Huiru Liu, Yaowu Fu, Lingli Zhang, Lei Tang, Yanmeng Liu & Qizhong Zhang

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  1. Tinglong Hou
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Hou, T., Xu, D., Cui, M. et al. The transcriptional factor GATA-4 negatively regulates Hsp70 transcription in Crassostrea hongkongensis. Mol Biol Rep 47, 7107–7114 (2020). https://doi.org/10.1007/s11033-020-05778-9

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