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Expression of endostatin mediated by a novel non-viral delivery system inhibits human umbilical vein endothelial cells in vitro

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Abstract

Ultrasound (US)-mediated microbubble destruction is recognized to have considerable potential for gene delivery, whereas, there is few report of its effect on enhancing liposomal transfection. In this study, we used pIRES2-EGFP/hES containing human endostatin (hES) cDNA as target gene to test the hypothesis that US exposure with microbubbles could improve liposomal transfection, and to investigate the possibility of intracellular delivery of ES gene using this method. Under the controlled US exposure condition with microbubbles, the plasmid:liposome was transferred into COS-7 cells. The transfection rate, the expression of endostatin and the inhibition effect of transfection-endostatin on endothelial cells were assessed. The results revealed that US-mediated microbubble destruction together with liposome could significantly enhance gene transfection without obvious cell damage. By this means, endostatin gene could be efficiently transferred into COS-7 cells and expressed. The transfection-endostatin could inhibit endothelial proliferation and migration, which suggests that the non-viral method might be useful in anti-angiogenesis therapy in the future.

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Acknowledgments

We especially thank Dr. Hongyan Ge, Lichun Pei, Hongsheng Liang, Shujun Zhang and Hua Chen for providing the excellent technical assistance throughout the experiment. This study was supported by a Grant for Key Program (No.GC06C413) from the Heilongjiang Provincial Science and Technology Committee, China.

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Authors and Affiliations

  1. Department of Ultrasound, First Affiliated Hospital of Harbin Medical University, 150001, Harbin, China

    Chunmei Zhang, Xueju Zhang, Chunbo Liu, Junfeng Wang & Changjun Wu

  2. Department of Biochemistry and Molecular Biology, Harbin Medical University, The Key Laboratory of Heilongjiang Provincial Biomedical Engineering, 150086, Harbin, China

    Xinghan Liu

  3. Department of Neurobiology, Harbin Medical University, 150086, Harbin, China

    Hulun Li & Jinghua Wang

Authors
  1. Chunmei Zhang
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  2. Xueju Zhang
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  3. Chunbo Liu
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  4. Junfeng Wang
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  5. Xinghan Liu
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  6. Hulun Li
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  7. Jinghua Wang
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  8. Changjun Wu
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Corresponding author

Correspondence to Changjun Wu.

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C. Zhang and X. Zhang contributed equally to this paper.

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Zhang, C., Zhang, X., Liu, C. et al. Expression of endostatin mediated by a novel non-viral delivery system inhibits human umbilical vein endothelial cells in vitro. Mol Biol Rep 37, 1755–1762 (2010). https://doi.org/10.1007/s11033-009-9600-9

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  • DOI: https://doi.org/10.1007/s11033-009-9600-9

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