Skip to main content
SpringerLink
Log in

Photochemical studies on the binding of an organic fluoride to bovine serum albumin

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

The interaction of fipronil (FPN), a pesticide containing fluorine, to bovine serum albumin (BSA) was studied by spectroscopy including fluorescence spectra, UV–Visible absorption, scattering spectra, circular dichroism (CD) spectra, synchronous and three-dimensional fluorescence spectra. The number of binding sites n and observed binding constant Kb was measured by fluorescence quenching method. The thermodynamic parameters ΔH, ΔG, ΔS at different temperatures were calculated and the results indicate that hydrophobic forces played major role in the reaction. The distance r between donor (BSA) and acceptor (FPN) was obtained according to the Förster theory of non-radiation energy transfer. The structural change of BSA molecules with addition of FPN was analyzed and the results may be helpful to biologists, chemists and therapeutists.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  1. Hu YJ, Liu Y, Sun TQ, Bai AM, LÜ JQ, PIA ZB (2006) Binding of anti-inflammatory drug cromolyn sodium to bovine serum albumin. J Biol Macromol 39:280–285. doi:10.1016/j.ijbiomac.2006.04.004

    Article  CAS  Google Scholar 

  2. Ashoka S, Seetharamappa J, Kandagal PB, Shaikh SMT (2006) Investigation of the interaction between trazodone hydrochloride and bovine serum albumin. J Lumin 121:179–186. doi:10.1016/j.jlumin.2005.12.001

    Article  CAS  Google Scholar 

  3. Purohit G, Sakthivel T, Florence AT (2003) The interaction of cationic dendrons with albumin and their diffusion through cellulose membranes. Int J Pharm 254:37–41. doi:10.1016/S0378-5173(02)00679-8

    Article  CAS  PubMed  Google Scholar 

  4. Mei P, Zhang YZ, Zhang XP, Yan CX, Zhang H, Liu Y (2008) Spectroscopic investigation of the interaction between copper (II) 2-oxo-propionic acid salicyloyl hydrazone complex and bovine serum albumin. Biol Trace Elem Res 124:269–282. doi:10.1007/s12011-008-8147-2

    Article  CAS  PubMed  Google Scholar 

  5. Carter DC, Ho JX (1994) The structure of serum albumin. Adv Protein Chem 45:153–203. doi:10.1016/S0065-3233(08)60640-3

    Article  CAS  PubMed  Google Scholar 

  6. Rosso SB, Gonzalez M, Bagatolli LA, Duffard RO, Fidelio GD (1998) Evidence of a strong interaction of 2, 4-dichlorophenoxyacetic acid herbicide with human serum albumin. Life Sci 63:2343–2351. doi:10.1016/S0024-3205(98)00523-2

    Article  CAS  PubMed  Google Scholar 

  7. Lakowicz JR (1999) Principles of fluorescence spectroscopy, 2nd edn. Plemum Press, New York, pp 13–16

    Google Scholar 

  8. Romanini D, Avalle G, Farruggia B, Nerli B, Pico G (1998) Spectroscopy features of the binding of polyene antibiotics to human serum albumin. Chem Biol Interact 115:247–260. doi:10.1016/S0009-2797(98)00075-1

    Article  CAS  PubMed  Google Scholar 

  9. Gelamo EL, Silva CHTP, Imasato H, Tabak M (2002) Interaction of bovine and human serum albumins with ionic surfactants: spectroscopy and modeling. Biochim Biophys Acta 1594:84–99

    CAS  PubMed  Google Scholar 

  10. Zhang HX, Huang X, Mei P, Li KH, Tong JQ (2006) Studies on the interaction of tricyclazole with β-Cyclodextrin and human serum albumin by spectroscopy. J Fluoresc 16:287–294. doi:10.1007/s10895-006-0087-7

    Article  CAS  PubMed  Google Scholar 

  11. Trigg GL (1960) Virtual binding and its relation to resonance scattering. Am J Phys 28(8):711–715. doi:10.1119/1.1935960

    Article  Google Scholar 

  12. Vaughan WM, Weber G (1970) Oxygen quenching of pyrenebutyric acid fluorescence in water: a dynamic probe of the microenvironment. Biochemistry 9:464–473. doi:10.1021/bi00805a003

    Article  CAS  PubMed  Google Scholar 

  13. Ross DP, Subramanian S (1981) Thermodynamics of protein association reactions: forces contributing to stability. Biochemistry 20:3096–3102. doi:10.1021/bi00514a017

    Article  CAS  PubMed  Google Scholar 

  14. Zhang HX, Huang X, Mei P, Gao S (2008) Interaction between Glyoxal-bis-(2-hydroxyanil) and bovine serum albumin in solution. J Solution Chem 37:631–640. doi:10.1007/s10953-008-9268-0

    Article  CAS  Google Scholar 

  15. Stryer L (1968) Fluorescence spectroscopy of proteins. Science 162:526–533. doi:10.1126/science.162.3853.526

    Article  CAS  PubMed  Google Scholar 

  16. Saha S, Samanta A (2002) Influence of the structure of amino group and polarity of the medium on the photo physical behavior of 4-amino-1, 8-naphthalamide derivatives. J Phys Chem A 106:4763–4771. doi:10.1021/jp013287a

    Article  CAS  Google Scholar 

  17. Förster T (1948) Intermolecular energy migration and fluorescence. Ann Phys 437:55–75. doi:10.1002/andp.19484370105

    Article  Google Scholar 

  18. Zhang HX, Huang X, Zhang M (2008) Spectral diagnostics of the interaction between pyridoxine hydrochloride and bovine serum albumin in vitro. Mol Biol Rep 35:699–705. doi:10.1007/s11033-007-9143-x

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Chemical and Environmental Engineering, Yangtze University, 434023, Jingzhou, Hubei, People’s Republic of China

    Jin-qiang Tong, Hua-xin Zhang & Ping Mei

  2. College of Chemistry and Pharmaceutical Engineering, Jingchu University of Technology, 448000, Jingmen, Hubei, People’s Republic of China

    Hua-xin Zhang

  3. College of Mechanical Engineering, Jingchu University of Technology, 448000, Jingmen, Hubei, People’s Republic of China

    Hong-mei Yang

Authors
  1. Jin-qiang Tong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hua-xin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hong-mei Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ping Mei
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hua-xin Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tong, Jq., Zhang, Hx., Yang, Hm. et al. Photochemical studies on the binding of an organic fluoride to bovine serum albumin. Mol Biol Rep 37, 1741–1747 (2010). https://doi.org/10.1007/s11033-009-9598-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-009-9598-z

Keywords

Search

Navigation