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Sequence identification, chromosomal mapping and tissue specific expression of the porcine paraoxonase 1 (PON1) gene

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Abstract

Serum paraoxonase (PON1) plays an important role in protecting low-density lipoprotein against oxidative modification. In this study, the full-length cDNA sequence (1,416 bp) of porcine PON1 was cloned by reverse transcription polymerase chain reaction and rapid amplification of cDNA ends. It was found to contain a 1,068 bp open reading frame encoding a deduced protein of 355 amino acids with a calculated molecular weight of 40.02 kDa. The genomic structure and sequence of porcine PON1 were also analyzed using a bacterial artificial chromosome clone of a Chinese Erhualian pig. The porcine PON1 gene contained nine exons and eight introns spanning approximately 29 kb, and was located on chromosome nine between microsatellite markers SWR915 and SW944 by IMpRH mapping. Porcine PON1 was highly expressed in kidney, followed by liver, lung and small intestine, expressed at an extremely low level in heart, and was hardly expressed in spleen, lymph, muscle of the anterior limb, cerebrum, fat, cerebellum or hypothalamus.

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Acknowledgments

We gratefully acknowledge Dr Ning Li, Dr Xiaoxiang Hu and Liming Ren for the provision of the Erhualian pig BAC library. We thank Dr Kui Li, Dr Zhonglin Tang, INRA and the University of Minnesota for the provision of the IMpRH panel. This work was supported by National “863” Program of China (No. 2007AA10Z161), Natural Science Foundation of China (No. 30771545), National “973” Program of China (No. 2006CB102101).

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Authors and Affiliations

  1. Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, 510642, Guangzhou, People’s Republic of China

    Shuihua Xie, Jiaqi Li, Chong Wang & Hao Zhang

  2. School of Life Science, Sun Yat-Sen University, 510275, Guangzhou, People’s Republic of China

    Yaosheng Chen & Delin Mo

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  1. Shuihua Xie
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  2. Jiaqi Li
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Correspondence to Jiaqi Li.

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Xie, S., Li, J., Chen, Y. et al. Sequence identification, chromosomal mapping and tissue specific expression of the porcine paraoxonase 1 (PON1) gene. Mol Biol Rep 37, 1347–1353 (2010). https://doi.org/10.1007/s11033-009-9514-6

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  • DOI: https://doi.org/10.1007/s11033-009-9514-6

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