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Partial molecular characterization, expression pattern, polymorphism and association analysis of porcine SKP2 gene

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Abstract

As a component of E3 ubiquitin protein ligases called SCFs, SKP2 protein belongs to a member of FBLs protein which is the biggest eukaryotic subfamily of F-BOX proteins with 12 members. In this study, we cloned and sequenced partial cDNA, intron 1 and intron 6 of porcine SKP2 gene. The partial cDNA is 1,402 bp long and has an open reading frame of 1,272 bp which encodes 424 putative amino acids. The deduced protein comprises a conserved F-BOX domain at position from the 90th to 140th amino acid. The phylogenetic tree indicated that porcine SKP2 has the closest genetic relationship with bovine SKP2 than other selected animal species. Quantitative RT-PCR analysis displayed that the tissue expression level of porcine SKP2 fluctuated remarkably in a large range, and it expressed in thymus with the highest level and in longissimus dorsi muscle with the lowest level. Two SNPs were identified, meanwhile, further polymorphism analysis with Cfr42I showed that AA genotype was in dominance absolutely among four kinds of unrelated Chinese indigenous miniature and one introduced Landrace pig breeds. In addition, association analysis with immune traits and blood parameters revealed that the SNP Cfr42I in intron 1 was significantly associated with red cell distribution width of neonate piglets at 0 day (P = 0.027).

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Acknowledgments

This work was supported by the National 973 Program (2006CB102105), National 863 Project (20060110Z1039), National Natural Science Foundation of China, the Key Project of National Natural Science Foundation of China (30830080), State Platform of Technology Infrastructure (2005DK21101), National Support Plan of China and Innovation Group Foundation of CAAS.

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, 430070, Wuhan, People’s Republic of China

    Y. Li, S. H. Zhao & K. M. Peng

  2. The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193, Beijing, People’s Republic of China

    Y. Li, S. L. Yang, Z. L. Tang, W. T. Cui, Y. L. Mu, M. X. Chu & K. Li

  3. College of Animal Science and Veterinary Medicine, Jiangxi Agricultural University, 330045, Nanchang, People’s Republic of China

    Y. Li

  4. South China Agricultural University, 510642, Guangzhou, People’s Republic of China

    Z. F. Wu

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  1. Y. Li
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Correspondence to K. M. Peng or K. Li.

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Li, Y., Yang, S.L., Tang, Z.L. et al. Partial molecular characterization, expression pattern, polymorphism and association analysis of porcine SKP2 gene. Mol Biol Rep 37, 1309–1317 (2010). https://doi.org/10.1007/s11033-009-9506-6

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  • DOI: https://doi.org/10.1007/s11033-009-9506-6

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