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The differential expression of peroxisome proliferators-activated receptors in various duck tissues

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Abstract

Peroxisome proliferators-activated receptors (PPARs) plays important role in regulate a variety of target genes which involves in lipid metabolism. In the present study, 1,430 bp of PPARα and 1,650 bp of PPARγ in Peking duck (Anas platyrhynchos) were cloned by reverse transcription (RT)-PCR. The cloned sequences of PPARα and PPARγ were highly conserved, although relevant changes with respect to human, mouse, chicken, and goose were identified. Real-time quantitative RT-PCR was used to determine if the PPARα and PPARγ were expressed in different tissues of male and female Peking ducks. The expression of PPARα and PPARγ were widely distributed in all examined tissues. The expression level of PPARα was highest in liver and lowest in kidney in male and female ducks. And it was highest in abdominal fat and lowest in small intestine of PPARγ in male and female ducks.

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Acknowledgments

This paper was supported by the Eleventh 5-year Program in China (Grant No. 2006BAD14B06-6-1) and the Doctor Fund of Northwest A&F University. The authors would like to thank Institute of Animal Science of Chinese Academy of Agriculture Science for collecting samples.

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Authors and Affiliations

  1. Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Yan Wu, Xiaolin Liu & Huilin Zhang

  2. Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Science, 430064, Wuhan, China

    Yan Wu & Hongwei Xiao

Authors
  1. Yan Wu
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  2. Xiaolin Liu
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  3. Hongwei Xiao
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  4. Huilin Zhang
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Correspondence to Xiaolin Liu.

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Wu, Y., Liu, X., Xiao, H. et al. The differential expression of peroxisome proliferators-activated receptors in various duck tissues. Mol Biol Rep 37, 1235–1240 (2010). https://doi.org/10.1007/s11033-009-9494-6

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  • DOI: https://doi.org/10.1007/s11033-009-9494-6

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