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PPAR-Alpha Cloning, Expression, and Characterization

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Peroxisome Proliferator-Activated Receptors (PPARs)

Part of the book series: Methods in Molecular Biology ((MIMB,volume 952))

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Abstract

Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems.

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Author information

Authors and Affiliations

  1. The University of Adelaide, Roseworthy, SA, Australia

    Suong N. T. Ngo

  2. Flinders university, Bedford Park, SA, Australia

    Ross A. McKinnon

Authors
  1. Suong N. T. Ngo
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  2. Ross A. McKinnon
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Corresponding author

Correspondence to Suong N. T. Ngo .

Editor information

Editors and Affiliations

  1. School of Pharmacy, University of Missouri-Kansas City, Charlotte Street 2464, Kansas City, 64108, USA

    Mostafa Z. Badr

  2. School of Pharmacy, University of Missouri-Kansas City, Charlotte Street 2464, Kansas City, 64108, Missouri, USA

    Jihan A. Youssef

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Ngo, S.N.T., McKinnon, R.A. (2013). PPAR-Alpha Cloning, Expression, and Characterization. In: Badr, M., Youssef, J. (eds) Peroxisome Proliferator-Activated Receptors (PPARs). Methods in Molecular Biology, vol 952. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-155-4_2

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  • DOI: https://doi.org/10.1007/978-1-62703-155-4_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-154-7

  • Online ISBN: 978-1-62703-155-4

  • eBook Packages: Springer Protocols

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