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Cloning, characterization and localization of CHS gene from blood orange, Citrus sinensis (L.) Osbeck cv. Ruby

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Abstract

Chalcone synthase (CHS) is involved in the biosynthesis of anthocyanin. In this study, a full-length DNA of CHS gene (named as CsCHS-bo) was cloned from the blood orange, Citrus sinensis (L.) Osbeck cv. Ruby. The gene was 1,512 bp in size containing an open reading frame (1,176 bp) encoding 391 amino acids. Comparative and bioinformatic analyses revealed that the deduced protein of CsCHS-bo was highly homologous to CHS from other plant species. The protein of CsCHS-bo had four CHS-specific conserved motifs and a CHS-family signature sequence GFGPG. Phylogenetic analysis indicated that the protein of CsCHS-bo was in a subgroup with CHS of Ruta Palmatum. The CsCHS-bo was localized to the chromosomes 2p, 4p and 6p by an improved fluorescence in situ hybridization technique, indicating that at least three copies of CsCHS-bo were present in the genome.

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Acknowledgments

The work was partially carried out in the Key Laboratory of MOE for Plant Development Biology in Wuhan University of China.

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Authors and Affiliations

  1. School of Life Sciences, South China Normal University, 510631, Guangzhou, People’s Republic of China

    Xu Lu, Wei Zhou & Feng Gao

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  1. Xu Lu
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  2. Wei Zhou
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  3. Feng Gao
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Corresponding author

Correspondence to Feng Gao.

Additional information

The novel nucleotide sequence data published here have been deposited in the EMBL/DDBJ/GenBank databases under accession number EU410483.

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Lu, X., Zhou, W. & Gao, F. Cloning, characterization and localization of CHS gene from blood orange, Citrus sinensis (L.) Osbeck cv. Ruby. Mol Biol Rep 36, 1983–1990 (2009). https://doi.org/10.1007/s11033-008-9408-z

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  • DOI: https://doi.org/10.1007/s11033-008-9408-z

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