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Molecular cloning, expression analysis and chromosome localization of the Tpt1 gene coding for the pig translationally controlled tumor protein (TCTP)

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Abstract

This work describes the cloning and structural analysis of a Tpt1 cDNA coding for the porcine translationally controlled tumor protein (TCTP) molecule and its expression in porcine cells and tissues. Pig Tpt1 cDNA is 842-pb long that displays typical features of translationally controlled mRNAs, including a 5′-UTR containing a 5′-terminal oligopyrimidine tract (5′-TOP), and a 3′-UTR with a high CG-content and one AU rich element (ARE). Both 5′-UTR and 3′-UTR are highly conserved when they are compared with those of other mammals. The pig Tpt1 cDNA contains a 516-b open reading frame that encodes a predicted TCTP protein composed of 172 amino acids that exhibits extensive conservation compared with TCTP sequences from other species and a common structural feature with all the other TCTP proteins analyzed in mammals. Expression analysis demonstrated that Tpt1 mRNA is ubiquitously expressed in normal porcine tissues and cells, showing a higher expression in spleen, lymph nodes and lung, and a lower one in skin and heart. The pig Tpt1 gene localizes on the porcine chromosome 11, region p11.

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Abbreviations

ARE:

AU rich element

DIG:

Digoxigenin

DMSO:

Dimethylsulphoxide

M-MLV:

Murine Moloney leukemia virus

PBL:

Peripheral blood lymphocyte

PBMC:

Peripheral blood mononuclear cell

PBS:

Phosphate buffered saline

SDS:

Sodium dodecylsulfate

Tpt1 :

Translationally controlled tumor gene

TCTP:

Translationally controlled tumor protein

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Acknowledgments

We are grateful to M. Yerle and D. Milan for providing the panel of irradiated pig/hamster somatic hybrid cells. The authors thanks to Reyes Álvarez (Universidad de Córdoba, Spain) and Jordi Estellé (Universidad Autónoma de Barcelona, Spain) for excellent technical assistance. This work has been founded by the National R&D Program Grant of the Spanish Ministry of Education and Science (AGL2002-00529and AGL2005-01561). AJM is a postdoctoral research fellow supported by an EADGENE fellowships. NY and GE are predoctoral research fellows of the University of Córdoba, Spain. JJG was a recipient of a “Ramón y Cajal” grant of the Spanish Ministry of Education and Science.

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Authors and Affiliations

  1. Department of Genetics, University of Córdoba, Campus de Rabanales, 14014, Córdoba, Spain

    Noemí Yubero, Gloria Esteso, Luis Morera, Juan J. Garrido & Manuel Barbancho

  2. Departamento de Ciencia Animal y de los Alimentos, Facultad de Veterinaria, Universidad Autónoma de Barcelona, 08193, Bellaterra, Spain

    Henry Cardona

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  1. Noemí Yubero
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Correspondence to Manuel Barbancho.

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Yubero, N., Esteso, G., Cardona, H. et al. Molecular cloning, expression analysis and chromosome localization of the Tpt1 gene coding for the pig translationally controlled tumor protein (TCTP). Mol Biol Rep 36, 1957–1965 (2009). https://doi.org/10.1007/s11033-008-9405-2

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