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Molecular characterization of Jatropha genetic resources through inter-simple sequence repeat (ISSR) markers

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Abstract

The genetic diversity among eight Jatropha species and three Jatropha curcas accessions were analyzed using ISSR-PCR. Nine ISSR primers generated reproducible amplification banding pattern of 61 polymorphic bands out of 64 scored accounting for 98.14% polymorphism across the species. The ISSR primers viz., I1, I2, I3, I4, I5, I6, I7 and I10 generated 100% polymorphic patterns. Jaccard’s coefficient of similarity varied from 0.346 to 0.807, indicative of high level of genetic variation among the genotypes studied. The UPGMA cluster analysis indicated three distinct clusters, one comprising all accessions of J. curcas L (TNMJ1, TNMJ 22 and TNMJ 23), while second included four species viz., J. tanjorensis J. L. Ellis et Saroja., J. gossypifolia L., J. podagrica Hook and J. maheshwarii Subrum and M.P. Nayer and the third cluster included another four species viz., J. villosa Wight J. multifida L., J. integerrima Jacq and J. glandulifera Roxb. The overall grouping pattern of clustering corresponds well with principal component analysis (PCA) confirming patterns of genetic diversity observed among the species. So far, there are no reports on the molecular diversity of the Jatropha species through ISSR marker. This study provides valid guidelines for collection, conservation and characterization of Jatropha genetic resources and also for further breeding programme towards biodiesel production.

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References

  1. Anonymous (1959) The wealth of India. Raw Material, vol V. H-K, Ed, pp 293–295

  2. Banerji R, Chowdhry AR, Misra G, Sunarsanam G, Verma SC, Srivastava GS (1985) Jatropha seed oils for energy. Biomass 8:277–282. doi:10.1016/0144-4565(85)90060-5

    Article  CAS  Google Scholar 

  3. Blair MW, Panaud O, McCouch SR (1999) Inter-simple sequence repeats (ISSR) amplification for analysis of micro satellite motif frequency and fingerprinting in rice (Oryza sativa L.). Theor Appl Genet 98:780–792. doi:10.1007/s001220051135

    Article  CAS  Google Scholar 

  4. Chandhari DC, Joshi DN (1999) Jatropha curcas a multipurpose species for economic property and wasteland development. Adv Plant Sci Res India 9:35–39

    Google Scholar 

  5. Charters YM, Wilkinsion MJ (2000) The use of self-pollinatated progenies as “in-groups” for the genetic characterization of cocoa germplasm. Theor Appl Genet 100:160–166. doi:10.1007/PL00002903

    Article  Google Scholar 

  6. Dehgan B (1984) Phylogenetic significance of interspecific hybridization in Jatropha (Euphobiaceae). Syst Bot 9:467–478. doi:10.2307/2418796

    Article  Google Scholar 

  7. Dellaporta SL, Wood J, Hick JB (1983) A plant DNA minipreparation; version II. Plant Mol Biol Rep 1:19–21. doi:10.1007/BF02712670

    Article  CAS  Google Scholar 

  8. Fang DQ, Roose ML (1997) Identification of closely related citrus cultivars with inter-simple sequence repeat makers. Theor Appl Genet 95:408–417. doi:10.1007/s001220050577

    Article  CAS  Google Scholar 

  9. Ganesh Ram S, Parthiban KT, Senthil Kumar R (2008) Genetic diversity among Jatropha species as revealed by RAPD markers. Genet Resour Crop Evol 55(6):803–809. doi:10.1007/s10722-007-9285-7

    Article  Google Scholar 

  10. Ginwal HS, Phartyal SS, Rawat PS, Srivastava RL (2005) Seed source variation in morphology, germination and seedling growth of Jatropha crucas Linn. in central India. Silvae Genet 54(2):76–80

    Google Scholar 

  11. Gonzalez A, Coulson A, Brettell R (2002) Development of DNA markers (ISSRs) in mango. Acta Hortic 575:139–143

    CAS  Google Scholar 

  12. Hao G, Lee DH, Lee JS, Lee NS (2002) A study of taxonomical relationships among species of Korean Allium sect. Sacculiferum (Alliaceae) and related species using inter-simple sequence repeat (ISSR) markers. Bot Bull Acad Sin 43:63–68

    CAS  Google Scholar 

  13. Heller J (1996) Physic nut- Jatropha curcas L. Promoting the conservation and use of underutilized and neglected crop. International Plant Genetic Resources Institute, Rome

    Google Scholar 

  14. Hong YP, Kim MJ, Hong KN (2003) Genetic diversity in natural populations of two geographic isolates of Korean black raspberry. J Hortic Sci Biotechnol 78:350–354

    Google Scholar 

  15. Jaccard P (1908) Nouvelles recherches sur la distribution florale. Bull Soc Vaud Nat 44:223–270

    Google Scholar 

  16. Lai JA, Yang WC, Hsiao JY (2001) An assessment of genetic relationships in cultivated tea clones and native wild tea in Taiwan using RAPD and ISSR markers. Bot Bull Acad Sin 42:93–100

    CAS  Google Scholar 

  17. Martin G, Mayeux A (1985) Curcas oil (Jatropha curcas L.): a possible fuel. Agric Trop 9:73–75

    Google Scholar 

  18. Moreno S, Martin JP, Oritz JM (1998) Inter-simple sequence repeats PCR for characterization of closely related grapevine germplasm. Euphytica 101:117–125. doi:10.1023/A:1018379805873

    Article  CAS  Google Scholar 

  19. Nagaoka T, Ogihara Y (1997) Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor Appl Genet 94:597–602. doi:10.1007/s001220050456

    Article  CAS  Google Scholar 

  20. Nei M, Tajima F, Tateno Y (1983) Accuracy of estimated phylogenetic tres from molecular date. II. Gene frequency data. J Mol Evol 19:153–170. doi:10.1007/BF02300753

    Article  PubMed  CAS  Google Scholar 

  21. Openshaw K (2000) A review of Jatropha curcas. An oil plant of unfulfilled promise. Biomass Bioenergy 19:1–15. doi:10.1016/S0961-9534(00)00019-2

    Article  Google Scholar 

  22. Rohlf FJ (2002) NTSYS-pc: numerical taxonomy system Ver 2.1. Exeter Publishing Ltd., Setauket, New York

  23. Ruas PM, Ruas CF, Rampim L, Carvalho VP, Ruas EA, Sera T (2003) Genetic relationship in Coffea species and parentage determination of inter-specific hybrids using ISSR (inter-simple sequence repeat) markers. Genet Mol Biol 26:319–327. doi:10.1590/S1415-47572003000300017

    Article  CAS  Google Scholar 

  24. Sneath PHA, Sokal RR (1973) Numerical taxonomy. Freeman Press, San Francisco, CA, USA

    Google Scholar 

  25. Sujatha M (1996) Genetic and tissue culture studies in Castor (Ricinus communis L.) and related genera. Ph.D. Dissertation, Osmania Univ., Hyderabad, India

  26. Sujatha M, Makkar HPS, Becker K (2005) Shoot bud proliferation from axillary nodes and leaf sections of non-toxin Jatropha curcas L. Plant Growth Regul 47:83–90. doi:10.1007/s10725-005-0859-0

    Article  CAS  Google Scholar 

  27. Takeda Y (1982) Development study on Jatropha curcas oil as a possible substitute for diesel engine oil in Thailand. J Agric Assoc China 120:1–8

    Google Scholar 

  28. Wolfe AD, Xiang QY, Kephart SR (1998) Assessing hybridization in natural populations of Penstemon (Scrophulariaceae) using hypervariable inter simple sequence repeat (ISSR) bands. Mol Ecol 7:1107–1125. doi:10.1046/j.1365-294x.1998.00425.x

    Article  PubMed  CAS  Google Scholar 

  29. Yasodha R, Kathirvel M, Sumathi R, Gurumurthi K, Archak Sunil, Nagraju J (2004) Genetic analysis of casuarina using ISSR and FISSR markers. Genetica 122:161–172. doi:10.1023/B:GENE.0000040938.13344.70

    Article  PubMed  CAS  Google Scholar 

  30. Zobel BJ, Talbert J (1984) Applied tree improvement. Wiley, New York 503 pp

    Google Scholar 

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Acknowledgments

The authors profusely thank DBT (Department of Biotechnology, India), New Delhi, for funding the project on “Breeding and Biotechnological Approaches for the Improvement of Jatropha Genetic resources”. The help rendered by Dr. S. Ganesh Ram, Dr. P. Hemalatha, Dr. S. Hariharan, Dr. Chezhiyan and Sundaramoorthi, TNAU, Coimbatore, India for their technical support is duly acknowledged.

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  1. Forest College and Research Institute, Mettupalayam, Tamil Nadu, 641 301, India

    R. Senthil Kumar, K. T. Parthiban & M. Govinda Rao

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  1. R. Senthil Kumar
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  2. K. T. Parthiban
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  3. M. Govinda Rao
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Correspondence to R. Senthil Kumar.

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Senthil Kumar, R., Parthiban, K.T. & Govinda Rao, M. Molecular characterization of Jatropha genetic resources through inter-simple sequence repeat (ISSR) markers. Mol Biol Rep 36, 1951–1956 (2009). https://doi.org/10.1007/s11033-008-9404-3

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  • DOI: https://doi.org/10.1007/s11033-008-9404-3

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