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Expression and sequence variation of the cucumber Por gene in the synthesized allotetraploid Cucumis × hytivus

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Abstract

The molecular evolution of the cucumber Por gene in early generations of the synthesized allotetraploid Cucumis × hytivus was investigated. The results from gene expression analysis showed that the cucumber Por gene was silenced in the S1 generation, and re-activated in the S2 generation. In the S3 and S4 generations, the transcripts remained activated but sequence changes were observed. Further analysis indicated that base substitutions, including two transitions and one transversion, occurred in the S1 and S3 generation, respectively, and in the S3 generation, an intron was found to be retained in the transcript. This indicates allopolyploidy induced rapid silencing and mutation of the cucumber Por gene. Further, gene mutations such as base substitution and intron retention are modes of evolution for duplicated genes in newly formed polyploids.

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References

  1. Osborn TC, Pires JC, Birchler JA, Auger DL, Chen ZJ, Lee HS, Comai L, Madlung A, Doerge RW, Colot V, Martienssen RA (2003) Understanding mechanisms of novel gene expression in polyploids. Trends Genet 19:141–147. doi:10.1016/S0168-9525(03)00015-5

    Article  PubMed  CAS  Google Scholar 

  2. McClintock B (1984) The significance of responses of the genome to challenge. Science 226:792–801. doi:10.1126/science.15739260

    Article  PubMed  CAS  Google Scholar 

  3. Comai L, Tyagi AP, Winter K, Holmes-Davis R, Reynolds SH, Stevens Y, Byers B (2000) Phenotypic instability and rapid gene silencing in newly formed Arabidopsis allotetraploids. Plant Cell 12:1551–1568

    Article  PubMed  CAS  Google Scholar 

  4. Madlung A, Masuelli RW, Watson B, Reynolds SH, Davison J, Comai L (2002) Remolding of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids. Plant Physiol 129:733–746. doi:10.1104/pp.003095

    Article  PubMed  CAS  Google Scholar 

  5. Wendel JF (2000) Genome evolution in polyploids. Plant Mol Biol 42:225–249. doi:10.1023/A:1006392424384

    Article  PubMed  CAS  Google Scholar 

  6. Levy AA, Feldman M (2004) Genetic and epigenetic reprogramming of the wheat genome upon allopolyploidy. Biol J Linn Soc Lond 82:607–613. doi:10.1111/j.1095-8312.2004.00346.x

    Article  Google Scholar 

  7. Chen JF, Kirkbride JJ (2000) A new synthetic species of Cucumis (Cucurbitaceae) from interspecific hybridization and chromosome doubling. Brittonia 52:315–319. doi:10.2307/2666583

    Article  Google Scholar 

  8. Chen JF, Staub JE, Adelberg J, Lewis S, Kunlle B (2002) Synthesis and preliminary characterization of a new species (Amphidiploid) in Cucumis. Euphytica 123:315–322. doi:10.1023/A:1015095430624

    Article  Google Scholar 

  9. Chen JF, Staub J, Qian CH, Luo XD, Zhuang FY (2003) Reproduction and cytogenetic characterization of interspecifichybrids from Cucumis hystix chakr. × C. sativus L. Theor Appl Genet 106:688–695

    PubMed  CAS  Google Scholar 

  10. Chen LZ, Lou QF, Zhuang Y, Chen JF, Zhang XQ, Wolukau JN (2007) Cytological diploidization and rapid genome changes of the newly synthesized allotetraploids Cucumis × hytivus. Planta 225:603–614. doi:10.1007/s00425-006-0381-2

    Article  PubMed  CAS  Google Scholar 

  11. VonWettstein D, Gough S, Kannangara CG (1995) Chlorophyll biosynthesis. Plant Cell 7:1039–1057

    Article  CAS  Google Scholar 

  12. Reinbothe S, Reinbothe C (1996) The regulation of enzymes involved in chlorophyll biosynthesis. Eur J Biochem 237:323–343. doi:10.1111/j.1432-1033.1996.00323.x

    Article  PubMed  CAS  Google Scholar 

  13. Reinbothe S, Reinbothe C (1996) Regulation of chlorophyll biosynthesis in angiosperms. Plant Physiol 111:1–7

    PubMed  CAS  Google Scholar 

  14. Fusada N, Masuda T, Kuroda H, Shiraishi T, Shimada H, Ohta H, Takamiya K (2000) NADPH-protochlorophyllide oxidoreductase in cucumber is encoded by a single gene and its expression is transcriptionally enhanced by illumination. Photo Res 64:147–154. doi:10.1023/A:1006418608647

    Article  CAS  Google Scholar 

  15. Kuroda H, Masuda T, Ohta H, Shioi Y, Takamiya K (1995) Light-enhanced gene expression of NADPH-protochlorophyllide oxidoreductase in cucumber. Biophys Biochem Res Commun 210:306–310. doi:10.1006/bbrc.1995.1662

    Google Scholar 

  16. Murray HG, Thompson WF (1980) Rapid isolation of higher weight DNA. Nucleic Acids Res 8:4321. doi:10.1093/nar/8.19.4321

    Article  PubMed  CAS  Google Scholar 

  17. Lee HS, Chen ZJ (2001) Protein-coding genes are epigenetically regulated in Arabidopsis polyploids. Proc Natl Acad Sci USA 98:6753–6758. doi:10.1073/pnas.121064698

    Article  PubMed  CAS  Google Scholar 

  18. Kashkush K, Feldman M, Levy AA (2002) Gene loss, silencing and activation in newly synthesized wheat allopolyploid. Genetics 160:1651–1659

    PubMed  CAS  Google Scholar 

  19. Kashkush K, Feldman M, Levy AA (2003) Transcriptional activation of retrotransposons alters the expression of adjacent genes in wheat. Nat Genet 33:102–106. doi:10.1038/ng1063

    Article  PubMed  CAS  Google Scholar 

  20. He P, Friebe BR, Gill BS, Zhou JM (2003) Allopolyploidy alters gene expression in the highly stable hexaploid wheat. Plant Mol Biol 52:401–414. doi:10.1023/A:1023965400532

    Article  PubMed  CAS  Google Scholar 

  21. Adams KL, Cronn R, Percifield R, Wendel JF (2003) Genes duplicated by Polyploid show unequal contributions to the transcriptome and organ-specific reciprocal silencing. Proc Natl Acad Sci USA 100:4649–4654. doi:10.1073/pnas.0630618100

    Article  PubMed  CAS  Google Scholar 

  22. Adams KL, Percifield R, Wendel JF (2004) Organ-specific silencing of duplicated genes in a newly synthesized cotton allotetraploid. Genetics 168:2217–2226. doi:10.1534/genetics.104.033522

    Article  PubMed  CAS  Google Scholar 

  23. Chen ZJ, Pikaard CS (1997) Epigenetic silencing of RNA polymerase I transcription: a role for DNA methylation and histon modification in nucleolar domince. Genes Dev 11:2124–2136. doi:10.1101/gad.11.16.2124

    Article  PubMed  CAS  Google Scholar 

  24. Comai L (2000) Genetic and epigenetic interactions in allopolyploid plants. Plant Mol Biol 43:387–399. doi:10.1023/A:1006480722854

    Article  PubMed  CAS  Google Scholar 

  25. Adams KL, Wendel JF (2004) Exploring the genomic mysteries of polyploidy in cotton. Biol J Linn Soc Lond 82:573–581. doi:10.1111/j.1095-8312.2004.00342.x

    Article  Google Scholar 

  26. Pires JC, Gaeta RT, Leon EJ, Zhao J, Schranz ME, Quijada PA, Lukens LN, Osborn TC (2004) Flowering time divergence and genomic rearrangements in resynthesized Brassica polyploids (Brassicaceae). Biol J Linn Soc Lond 82:675–688. doi:10.1111/j.1095-8312.2004.00350.x

    Article  Google Scholar 

  27. Dias AP, Braun EL, McMullen MD, Grotewold E (2003) Recently duplicated maize R2R3 Myb genes provide evidence for distinct mechanisms of evolutionary divergence after duplication. Plant Physiol 131:610–620. doi:10.1104/pp012047

    Article  PubMed  CAS  Google Scholar 

  28. Czarnecka E, Nagao RT, Key JL (1988) Characterization of Gmhsp26-a, a stress gene encoding a divergent heat shock protein of soybean: heavy-metal-induced inhibition of intron processing. Mol Cell Biol 8:1113–1122

    PubMed  CAS  Google Scholar 

  29. Grotewold E, Athrma P, Peterson T (1991) Alternatively spliced products of the maize P gene encode proteins with homology to the DNA-binding domain of myb-like transcription factors. Proc Natl Acad Sci USA 88:4587–4591. doi:10.1073/pnas.88.11.4587

    Article  PubMed  CAS  Google Scholar 

  30. Nash J, Walbot V (1992) Bronze-2 gene expression and intron splicing patterns in cells and tissues of Zea mays L. Plant Physiol 100:464–471

    Article  PubMed  CAS  Google Scholar 

  31. Golvkin M, Reddy ASN (1996) Structure and expression of a plant U1 snRNP 70 K pre-mRNAs produces two different transcripts. Plant Cell 8:1421–1435

    Article  Google Scholar 

  32. Yang HJ, Wang ZY, Zhang JL, Hong MM (2002) First intron retention in part transcripts of OsEBP-89 gene in tissues of rice. Chin Sci Bull 47(5):394–397. doi:10.1360/02tb9092

    Article  Google Scholar 

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Acknowledgments

This research was partially supported by the Key Program (30830079) and the General Program 30671419 and 30700541 from the National Natural Science Foundation of China; the ‘863’ Programs (2008AA10Z150, 2006AA10Z1A8, 2006AA100108); the ‘111’ Project (B08025); the ‘973’ Program and the National Supporting Programs (2006BAD13B06, 2006BAD01A7-5-11) from the Ministry of Science and Technology of China; PSRF of Pickle Packer International co.; and the Ph.D. Funding (20050307009) from the Ministry of Education of the People’s Republic of China. We thank Michael Mazourek for assistance in the preparation of the manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China

    Yong Zhuang & Jin-Feng Chen

  2. College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, WI, 53706, USA

    Molly Jahn

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  1. Yong Zhuang
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  2. Jin-Feng Chen
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Correspondence to Jin-Feng Chen.

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Zhuang, Y., Chen, JF. & Jahn, M. Expression and sequence variation of the cucumber Por gene in the synthesized allotetraploid Cucumis × hytivus . Mol Biol Rep 36, 1725–1731 (2009). https://doi.org/10.1007/s11033-008-9374-5

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  • DOI: https://doi.org/10.1007/s11033-008-9374-5

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