Abstract
Cold shock domain proteins (CSPs), initially identified in Escherichia coli, have been demonstrated to play a positive role in cold resistance. Previous studies in wheat, rice, and Arabidopsis have indicated the functional conservation of CSPs in cold resistance between bacteria and higher plants. However, the biological functions of PbrCSPs in pear pollen tubes, which represent the fragile reproductive organs highly sensitive to low temperature, remain largely unknown. In this study, a total of 22 CSPs were identified in the seven Rosaceae species, with a focus on characterizing four PbrCSPs in pear (Pyrus bretschneideri Rehder). All four PbrCSPs were structurally conserved and responsive to the abiotic stresses, such as cold, high osmotic, and abscisic acid (ABA) treatments. PbrCSP1, which is specifically expressed in pear pollen tubes, was selected for further research. PbrCSP1 was localized in both the cytoplasm and nucleus. Suppressing the expression of PbrCSP1 significantly inhibited the pollen tube growth in vitro. Conversely, overexpression of PbrCSP1 promoted the growth of pear pollen tubes under the normal condition and, notably, under the cold environment at 4 °C. These findings highlight an essential role of PbrCSP1 in facilitating the normal growth and enhancing cold resistance in pear pollen tubes.
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Acknowledgements
We thank the Bioinformatics Center of Nanjing Agricultural University for supporting bioinformatic analysis. We thank Dr. Yuehua Ma (Central laboratory of College of Horticulture, Nanjing Agricultural University) for assistance in using laser scanning confocal microscope LSM800. We thank Dr. Barend H.J. de Graaf (Cardiff University) for offering us the vector NTP303:GFP.
Funding
This work was financially supported through grants from the open funds of the Jiangsu Agricultural Science and Technology Innovation Fund (CX(22)3161), National Natural Science Foundation of China (32172543, 32102358), the guidance foundation of Hainan Institute of Nanjing Agricultural University (NAUSY-MS08), Fundamental Research Fund for the Central Universities (YDZX2023019), National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops (Horti-KF-2023–05), Xinjiang Tianshan Innovation Team Foundation (2023D14015), Ningbo Key Laboratory of Characteristic Horticultural Crops in Quality Adjustment and Resistance Breeding (NBYYL2023001), Priority Academic Program Development of Jiangsu Higher Education Institutions, and Earmarked Fund for China Agriculture Research System (CARS-28), Natural Science Foundation of Jiangsu Province (BK20210394). This study was supported by the High-performance Computing Platform of the Bioinformatics Center, Nanjing Agricultural University.
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All authors contributed to the research planning and experimental designs. Juyou Wu, Shaoling Zhang, and Peng Wang initiated and supervised the project. Xiaoxuan Zhu, Chao Tang, and Ting Zhang performed the experiments and analyzed the data. Xiaoxuan Zhu and Chao Tang wrote the manuscript. All authors read and revised the manuscript.
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Zhu, X., Tang, C., Zhang, T. et al. PbrCSP1, a pollen tube–specific cold shock domain protein, is essential for the growth and cold resistance of pear pollen tubes. Mol Breeding 44, 18 (2024). https://doi.org/10.1007/s11032-024-01457-w
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DOI: https://doi.org/10.1007/s11032-024-01457-w