Abstract
Frogeye leaf spot is a yield-reducing disease of soybean caused by the pathogen Cercospora sojina. Rcs3 has provided durable resistance to all known races of C. sojina since its discovery in the cultivar Davis during the 1980s. Using a recombinant inbred line population derived from a cross between Davis and the susceptible cultivar Forrest, Rcs3 was fine-mapped to a 1.15 Mb interval on chromosome 16. This single locus was confirmed by tracing Rcs3 in resistant and susceptible progeny derived from Davis, as well as three near-isogenic lines. Haplotype analysis in the ancestors of Davis indicated that Davis has the same haplotype at the Rcs3 locus as susceptible cultivars in its paternal lineage. On the basis of these results, it is hypothesized that the resistance allele in Davis resulted from a mutation of a susceptibility allele. Tightly linked SNP markers at the Rcs3 locus identified in this research can be used for effective marker-assisted selection.
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All data generated or analyzed during this study are included in this published article and its supplementary information files or are available from the corresponding author upon reasonable request.
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Acknowledgements
A special thanks to: Dr. Qijian Song, Beltsville Agricultural Research Station, USDA-ARS, Beltsville, MD for genotyping the near isogenic lines; Carol Picard for providing support in growing plants, pathogen cultures, and phenotyping. Thanks also go to Nicole Bachleda and Tatyana Nienow for providing technical lab assistance, and to Dale Wood, Earl Baxter, Brice Wilson, Brian Little, and Greg Gokalp for their help with developing populations. SM appreciates the support provided by the Roger and Cindy Boerma Excellence in Plant Breeding Award.
Funding
Funding for this research was provided by the University of Georgia Research Foundation, Georgia Agricultural Commodity Commission for Soybeans, and Georgia Seed Development. This research was also supported by the funds allocated to the Georgia Agricultural Experiment Stations.
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SM conducted experiments, analyzed the data, interpreted results, and drafted the manuscript; JB oversaw phenotyping and edited the manuscript; ZL conceived the project, interpreted results, and edited the manuscript. All authors read and approved the final manuscript.
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Supplementary file1 Comparison of the genomic region of Rcs3 among Wright-Rcs3, Thomas-Rcs3, Gordon-Rcs3, Dillon, Hagood, Cook, and Forrest × Davis RILs. Colored bars represent the region of Rcs3 based on that line/population. Scale bar at the bottom gives the physical position along chromosome 16 in the Williams82.a2 genome. (PDF 139 KB)
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McDonald, S.C., Buck, J.W. & Li, Z. Pinpointing Rcs3 for frogeye leaf spot resistance and tracing its origin in soybean breeding. Mol Breeding 43, 49 (2023). https://doi.org/10.1007/s11032-023-01397-x
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DOI: https://doi.org/10.1007/s11032-023-01397-x