A major QTL (qFT12.1) allele from wild soybean delays flowering time
- 296 Downloads
Soybean is highly sensitive to photoperiod. To improve the adaptability and productivity of soybean, it is essential to understand the molecular mechanisms regulating flowering time. To identify new flowering time QTLs, we evaluated a BC3F5 population consisting of 120 chromosome segment substitution lines (CSSLs) over 2 years under field conditions. CSSLs were derived from a cross between the cultivated soybean cultivar Jackson and the wild soybean accession JWS156-1, followed by continuous backcrossing using Jackson as the recurrent parent. Four QTLs (qFT07.1, qFT12.1, qFT12.2, and qFT19.1) were detected on three chromosomes. Of these, qFT12.1 showed the highest effect, accounting for 36.37–38.27% of the total phenotypic variation over 2 years. This QTL was further confirmed in the F7 recombinant inbred line population (n = 94) derived from the same cross (Jackson × JWS156-1). Analysis of the qFT12.1 BC3F5 residual heterozygous line RHL509 validated the allele effect of qFT12.1 and revealed that the recessive allele of qFT12.1 resulted in delayed flowering. Evaluating the qFT12.1 near-isogenic lines (NILs) under different growth conditions showed that NILs with the wild soybean genotype always showed later flowering than those with the cultivated soybean genotype. qFT12.1 was delimited to a 2703-kb interval between the markers BARCSOYSSR_12_0220 and BARCSOYSSR_12_0368 on chromosome 12. qFT12.1 may be a new flowering time gene locus in soybean.
KeywordsFlowering time QTL Wild soybean CSSLs
We thank Ms. Suzuki, Ms. Noda, and Ms. Kobayashi for assisting in experiments.
This study was financially supported in part by a scholarship from the China Scholarship Council to Dequan Liu.
- Buzzell RI, Voldeng HD (1980) Inheritance of insensitivity to long daylength. Soyb Genet Newsl 7:26–29Google Scholar
- Do TD, Chen HT, Hien VT, Hamwieh A, Yamada T, Sato T, Yan YL, Cong H, Shono M, Suenaga K, Xu DH (2016) Ncl synchronously regulates Na+, K+, and Cl– in soybean and greatly increases the grain yield in saline field conditions. Sci Rep 6:19147. https://doi.org/10.1038/srep19147 CrossRefPubMedPubMedCentralGoogle Scholar
- Grandillo S, Tanksley SD (2005) Advanced backcross QTL analysis: results and perspectives. In: Mugnozza GTS, Porceddu E, Pagnotta MA (ed) Genetics and breeding for crop quality and resistance, 1rd edn. Viterbo, Italy, pp 283–290Google Scholar
- Lu SJ, Zhao XH, Hu YL, Liu SL, Nan HY, Li XM, Fang C, Cao D, Shi XY, Kong LP, Su T, Zhang FG, Li SC, Wang Z, Yuan XH, Cober ER, Weller JL, Liu BH, Hou XL, Tian ZX, Kong FJ (2017) Natural variation at the soybean J locus improves adaptation to the tropics and enhances yield. Nat Genet 49:773–779CrossRefPubMedGoogle Scholar
- Xia ZJ, Watanabe S, Yamada T, Tsubokura Y, Nakashima H, Zhai H, Anai T, Sato S, Yamazaki T, Lü SX, Wu HY, Tabata S, Harada K (2012) Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1 that regulates photoperiodic flowering. Proc Natl Acad Sci 109:E2155–E2164CrossRefPubMedPubMedCentralGoogle Scholar
- Xin DW, Qi ZM, Jiang HW, Hu ZB, Zhu RS, Hu JH, Han HY, Hu GH, Liu CY, Chen QS (2016) QTL location and epistatic effect analysis of 100-seed weight using wild soybean (Glycine soja Sieb. & Zucc.) chromosome segment substitution lines. PLoS One 11:e0149380. https://doi.org/10.1371/journal.pone.0149380 CrossRefPubMedPubMedCentralGoogle Scholar
- Yamamoto Y, Sato E, Shimizu T, Nakamich N, Sato S, Kato T, Tabata S, Nagatani A, Yamashino T, Mizuno T (2003) Comparative genetic studies on the APRR5 and APRR7 genes belonging to the APRR1/TOC1 quintet implicated in circadian rhythm, control of flowering time, and early photomorphogenesis. Plant Cell Physiol 44:1119–1130CrossRefPubMedGoogle Scholar
- Zhou ZK, Jiang Y, Wang Z, Gou ZH, Lyu J, Li WY, Yu YJ, Shu LP, Zhao YJ, Ma YM, Fang C, Shen YT, Liu TF, Li CC, Li Q, Wu M, Wu YS, Dong Y, Wan WT, Wang X, Ding ZL, Gao YD, Xiang H, Zhu BG, Lee SH, Wang W, Tian ZX (2015a) Resequencing 302 wild and cultivated accessions identifies genes related to domestication and improvement in soybean. Nat Biotechnol 33:408–414CrossRefPubMedGoogle Scholar
- Zhou L, Wang SB, Jian JB, Geng QC, Wen QJ, Wu ZZ, Li GJ, Liu YQ, Dunwell JM, Zhang J, Feng JY, Niu Y, Zhang L, Ren WL, Zhang YM (2015b) Identification of domestication-related loci associated with flowering time and seed size in soybean with the RAD-seq genotyping method. Sci Rep 5:9350. https://doi.org/10.1038/srep09350 CrossRefPubMedPubMedCentralGoogle Scholar