Identification and characterization of two waterlogging responsive alcohol dehydrogenase genes (AdADH1 and AdADH2) in Actinidia deliciosa
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Previous study showed that two alcohol dehydrogenase genes (ADHs) were significantly upregulated in roots of Actinidia deliciosa after treatment with waterlogging using Illumina sequencing technology. Those two ADH genes, named AdADH1 and AdADH2, were isolated from A. deliciosa in this study. QRT-PCR analysis showed that AdADH1 and AdADH2 expression levels were significantly increased in A. deliciosa after treatment with waterlogging, NaCl, 4 °C, and heat stresses, but not ABA and drought stresses. The changes of AdADH1 and AdADH2 expression levels during waterlogging were much higher than those during other stresses. We examined the response to abiotic stresses in transgenic Arabidopsis lines in which the A. deliciosa AdADH1 and AdADH2 genes were introduced under the control of a constitutive promoter, respectively. Overexpression of A. deliciosa AdADH1 or AdADH2 in Arabidopsis could enhance waterlogging tolerance at five week old seedlings. However, the function of AdADH1 for waterlogging resistance was much better than that of AdADH2. Arabidopsis overexpressing AdADH1 gene could enhance the resistance to cold stress at five week old seedlings, but AdADH2 could not. Overexpression of AdADH1 gene enhances the resistance to salt stress at the stage of seed germination and in seedlings, and overexpression of AdADH2 gene enhances the resistance to salinity stress at the stage of seed germination but not in seedlings. However, transgenic Arabidopsis overexpressing AdADH1 or AdADH2 could not enhance to the osmotic stress at the stage of seed germination and in seedlings. These results indicated that A. deliciosa AdADH1 plays an important role in resistance to waterlogging, salinity, and cold stresses, but not drought stress, and AdADH2 plays an important role in resistance to waterlogging and salinity stresses, but not cold and drought stresses.
KeywordsAbiotic stresses Actinidia deliciosa AdADH1 AdADH2 Waterlogging Transgenic Arabidopsis
Cauliflower mosaic virus
Hypoxia responsive ERF1/2
Murashige and Skoog
Real time quantitative RT-PCR
This study was supported by the National Natural Science Foundation of China (NSFC) (31401854) and grants from the Natural Science Foundation of Jiangsu Province (G rants No BK20140760).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Cai BH, Zhang JY, Gao ZH, Qu SC, Tong ZG, Mi L, Qiao YS, Zhang Z (2008) An improved method for isolation of total RNA from the leavesof Fragaria spp. Jiangsu J Agric Sci 24:875–877Google Scholar
- Diab AA, Kantety RV, Ozturk NZ, Benscher D, Nachit MM, Sorrells ME (2008) Drought - inducible genes and differentially expressed sequence tags associated with components of drought tolerance in durum wheat. Sci Res Essay 3:9–26Google Scholar
- Liang Y, Yan J-p, Tan X-l (2012) Effects of salt-stress on expression of ADH3 and ALDH in radicle of rice (Oryza sativa). Hubei Agric Sci 51:2870–2872Google Scholar
- Rahman M, Grover A, Peacock W, Dennis E, Ellis M (2001) Effects of manipulation of pyruvate decarboxylase and alcohol dehydrogenase levels on the submergence tolerance of rice. Aust J Plant Physiol 28:1231–1241Google Scholar
- Senthil-Kumar M, Hema R, Suryachandra TR, Ramegowda HV, Gopalakrishna R, Rama N, Udayakumar M, Mysore KS (2010) Functional characterization of three water deficit stress-induced genes in tobacco and Arabidopsis: an approach based on gene down regulation. Plant Physiol Biochem 48:35–44CrossRefPubMedGoogle Scholar
- Zhang J-y, Qu S-c, Dong C, Gao Z-h, Qiao Y-s, Zhang Z (2010) Utility and construction of full-length cDNA library of Malus hupehensis post-introduced with salicylic acid. Acta Bot Boreal -Occident Sin 30:1527–1533Google Scholar
- Zhang J, Wang G, Huang S, Xuan J, Jia X, Guo Z (2015b) Functions of alcohol dehydrogenase family in abiotic stress responses in plants. Chin Agric Sci Bull 31:246–250Google Scholar