Abstract
Adzuki bean is an important food legume crop in East Asia. China is ranked as the number one country for its production and consumption. A large number of adzuki bean accessions are maintained in the Chinese national seed genebank. Tapping its genetic diversity may have potential in assisting breeding programs for new cultivar development and improvement. However, its genetic diversity was not well evaluated due to lack of sufficient DNA markers. For marker development, we initiated de novo transcriptome sequencing of adzuki bean. From sequence assembly, 65,950 unigenes were identified, and some of them were used for EST-SSR (eSSR) marker development. A set of 53 SSR markers were developed from adzuki bean, and 110 SSR (including 57 already available) markers were used for genotyping of 261 selected accessions. These adzuki bean accessions were classified into 10 clusters. The accessions from North China were well separated from the accessions from South China. The average polymorphic information content of genomic SSR (gSSR) markers and eSSR markers was 0.5081 and 0.2382, respectively. Results indicate that gSSR markers were more polymorphic than eSSR markers. These polymorphic eSSR and gSSR markers will be useful for assisting adzuki-bean-breeding programs and future germplasm curation.
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Change history
22 June 2018
The original version of this article unfortunately contained an error in the number of unigenes reported in the Result section of Molecular Breeding article, “Development of SSR markers and assessment of genetic diversity of adzuki bean in the Chinese germplasm collection” (Mol Breeding (2015) 35:191, DOI https://doi.org/10.1007/s11032-015-0383-5).
02 May 2017
An erratum to this article has been published.
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Acknowledgments
This study was funded by the Agricultural Science and Technology Innovation Program (ASTIP) in CAAS and the China Agriculture Research System (CARS-09) from the Ministry of Agriculture of China.
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Table 1
List of 261 genotypes used in this study and their inferred subpopulations (XLS 62 kb)
Table 2
Characteristics of 400 adzuki bean EST-SSR, 100 mung bean genomic SSR, and 57 adzuki bean genomic SSR markers in this study (XLS 196 kb)
Table 3
Summary statistics of genetic diversity for model-based groups (DOC 39 kb)
Table 4
Analysis of molecular variance (AMOVA) based on 110 SSR markers (DOC 28 kb)
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Chen, H., Liu, L., Wang, L. et al. Development of SSR markers and assessment of genetic diversity of adzuki bean in the Chinese germplasm collection. Mol Breeding 35, 191 (2015). https://doi.org/10.1007/s11032-015-0383-5
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DOI: https://doi.org/10.1007/s11032-015-0383-5