Genetic analysis reveals a novel locus in Vicia faba decoupling pigmentation in the flower from that in the extra-floral nectaries
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The genetics of the stipule spot pigmentation (SSP) in faba bean (Vicia faba L.) was studied using four inbred lines, of which Disco/2 was zero-tannin (zt2) with colourless stipule spots, ILB938/2 was normal-tannin (ZT2) with colourless stipule spots, and both Aurora/2 and Mélodie/2 were ZT2 with coloured stipule spots. Crosses Mélodie/2 × ILB 938/2, Mélodie/2 × Disco/2, ILB 938/2 × Aurora/2 and ILB 938/2 × Disco/2 (A, B, C and D, respectively) were prepared, along with reciprocals and backcrosses, and advanced through single-seed descent. All F1 hybrid plants had pigmented stipule spots, and in the F2 generation, the segregation ratio fit 3 coloured:1 colourless in crosses A, B and C and 9:7 in cross D. In the F3 generation, the ratio fit 5:3 in crosses A and C and 25:39 in cross D, and in the F4 generation, 9:7 in cross A. SSP was linked to the zero-tannin characteristics (white flower) only in cross B. The results show that coloured stipule spot is dominant to colourless and that colouration is determined by two unlinked complementary recessive genes. We propose the symbols ssp2 for the gene associated with zt2 in Disco/2 and ssp1 for the gene not associated with tannin content in ILB938/2. The novel ssp1 locus was mapped at F5 in cross ‘A’ using Medicago truncatula-derived single-nucleotide polymorphism and was on chromosome 1 of faba bean, in a well-conserved region of M. truncatula chromosome 5 containing some candidate Myb and basic helix–loop–helix transcription factor genes.
KeywordsStipule spot pigmentation Inheritance Vicia faba Synteny Medicago truncatula
HK expresses his gratitude to CIMO (the Centre for International Mobility) and the Emil Aaltonen Foundation (Emil Aaltosen Säätiö) for their financial support. The project was also supported by the University of Helsinki, Niemi-säätiö and the FP7 project Legume Futures (245216 CP-FP ‘Legume Futures: Legume supported cropping systems for Europe’). We thank Markku Tykkyläinen, Sanna M. Peltola and Sini Lindström, technical assistants of the glasshouses of the University of Helsinki, for their kind assistance during the experiments. We would like to thank Hany El Sayed for his help for microsatellite loci screening. We also thank Prof. Wolfgang Link (Georg-August-University, Göttingen, Germany) for providing the seeds of the parental lines.
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