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Detection of novel blast resistance genes, Pi58(t) and Pi59(t), in a Myanmar rice landrace based on a standard differential system

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Abstract

The use of broad-spectrum R genes is an effective way to achieve durable resistance against rice blast (Magnaporthe oryzae Couch, anamorph: Pyricularia oryzae Cavara) in rice (Oryza sativa L.). We previously surveyed the diversity of blast resistance in 948 rice varieties and found a Myanmar rice landrace, Haoru (International Rice Research Institute genebank acc. no. IRGC33090), with broad-spectrum resistance against the standard differential blast isolates. Here, we examined the genetic basis of Haoru’s broad-spectrum resistance by using the standard blast differential system consisting of the standard isolates and differential varieties. For genetic analysis, we used a BC1F1 population and BC1F2 lines derived from crosses of Haoru with a susceptible variety, US-2. Co-segregation analysis of the reaction pattern in the BC1F1 population against the 20 standard isolates suggested that Haoru harbors three R genes. By using bulk-segregant and linkage analysis, we mapped two of the three R genes on chromosomes 12 and 6, and designated them as Pi58(t) and Pi59(t), respectively. Pi58(t) and Pi59(t) were differentiated from other reported R genes using the standard differential system. The estimated resistance spectrum of Pi58(t) corresponded with that of Haoru, suggesting that Pi58(t) is primarily responsible for Haoru’s broad-spectrum resistance. In addition, Pi59(t) and the third gene were also proven to be new and useful genetic resources for studying and improving blast resistance in rice.

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Acknowledgments

This paper reports the results obtained under the IRRI–Japan Collaborative Research Project. We thank the Ministry of Agriculture, Forestry and Fisheries of Japan and the Ministry of Foreign Affairs of Japan for funding of the project. Y. K. acknowledges support from the Japan Society for the Promotion of Science (JSPS Research Fellowships for Young Scientists, 22-6429).

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Authors and Affiliations

  1. Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki, 305-8686, Japan

    Yohei Koide, Mary Jeanie Telebanco-Yanoria, Yoshimichi Fukuta & Nobuya Kobayashi

  2. International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines

    Yohei Koide, Mary Jeanie Telebanco-Yanoria & Nobuya Kobayashi

  3. Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki, 305-8518, Japan

    Nobuya Kobayashi

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  1. Yohei Koide
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  2. Mary Jeanie Telebanco-Yanoria
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  3. Yoshimichi Fukuta
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  4. Nobuya Kobayashi
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Correspondence to Nobuya Kobayashi.

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Koide, Y., Telebanco-Yanoria, M.J., Fukuta, Y. et al. Detection of novel blast resistance genes, Pi58(t) and Pi59(t), in a Myanmar rice landrace based on a standard differential system. Mol Breeding 32, 241–252 (2013). https://doi.org/10.1007/s11032-013-9865-5

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  • DOI: https://doi.org/10.1007/s11032-013-9865-5

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