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QTL analysis for resistance to bacterial wilt (Burkholderia caryophylli) in carnation (Dianthus caryophyllus) using an SSR-based genetic linkage map

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Abstract

Bacterial wilt (Burkholderia caryophylli (Burkholder) Yabuuchi et al.) is one of the most damaging diseases during carnation (Dianthus caryophyllus L.) cultivation in Japan. To find molecular markers for use in marker-assisted selection, we constructed a simple sequence repeat (SSR)-based genetic linkage map of carnation using an F2 population of 90 plants derived from a cross between a highly resistant line (85-11) and a susceptible cultivar (Pretty Favvare). To develop a large number of SSR markers, we constructed four new SSR-enriched genomic libraries and conducted expressed sequence tag analysis. We mapped 178 SSR loci into 16 linkage groups. The map covered 843.6 cM, with an average distance of 6.5 cM between two loci. This is the first report of a genetic linkage map based mainly on SSR markers in the genus Dianthus. Quantitative trait locus (QTL) analysis identified one locus for resistance to bacterial wilt in linkage group (LG) B4. The locus explained 63.0% of the phenotypic variance for resistance to bacterial wilt. The SSR markers CES1161 and CES2643 that were closest to the QTL were efficient markers for selecting lines with resistance derived from line 85-11. A positional comparison using SSR markers as anchor loci revealed that LG B4 corresponded to LG A6 in a previously constructed map. We found that the position of the resistance locus derived from line 85-11 was similar to that of the major resistance locus observed for a highly resistant wild species, Dianthus capitatus ssp. andrzejowskianus.

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Acknowledgments

This work was supported by the National Agriculture and Food Research Organization Research Project No. 211, “Establishment of Integrated Basis for Development and Application of Advanced Tools for DNA Marker-Assisted Selection in Horticultural Crops”.

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Authors and Affiliations

  1. National Agriculture and Food Research Organization (NARO), National Institute of Floricultural Science (NIFS), 2-1 Fujimoto, Tsukuba, Ibaraki, 305-8519, Japan

    M. Yagi & T. Onozaki

  2. National Center for Seeds and Seedlings, 2-2 Fujimoto, Tsukuba, Ibaraki, 305-0852, Japan

    T. Kimura

  3. National Agriculture and Food Research Organization (NARO), National Institute of Fruit Tree Science (NIFTS), 2-1, Fujimoto, Tsukuba, Ibaraki, 305-8605, Japan

    T. Yamamoto

  4. Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan

    S. Isobe & S. Tabata

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  1. M. Yagi
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  2. T. Kimura
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  3. T. Yamamoto
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  4. S. Isobe
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Correspondence to M. Yagi.

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Yagi, M., Kimura, T., Yamamoto, T. et al. QTL analysis for resistance to bacterial wilt (Burkholderia caryophylli) in carnation (Dianthus caryophyllus) using an SSR-based genetic linkage map. Mol Breeding 30, 495–509 (2012). https://doi.org/10.1007/s11032-011-9639-x

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