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The application of DNA marker and doubled-haploid technology for stacking multiple stem rust resistance genes in wheat

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Abstract

In wheat, the use of gene “pyramids” or “stacks” of major genes that confer resistance to all local strains of the fungal stem rust pathogen Puccinia graminis f. sp. tritici (Pgt) can increase durability of resistance where wheat cultivars with the single gene components are not widely deployed. Stacking two or more resistance genes becomes a breeding challenge, particularly when pathogen races that discriminate the genes are not available. The use of DNA markers and doubled-haploid technology provides a route for producing lines homozygous for multiple resistance genes. We have applied this approach to produce gene pyramids of two or more of the stem rust resistance genes Sr24 and new sources of SrR, Sr31 and Sr26 on reduced alien chromatin in the genetic backgrounds of Westonia and Pavon wheat. These genes, which are all derived from “alien” sources (SrR and Sr31 from rye, Sr24 and Sr26 from Agropyron elongatum) each provide resistance to all currently known pathotypes of Pgt in Australia, and SrR and Sr26 also provide resistance against all the variants of stem rust race Ug99 (TTKS group).

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References

  • Anugrahwati DR (2006) Isolation, characterization and quality testing of 1DS/1RS wheat-rye recombinants. Ph.D. thesis. Discipline of plant and pest science, school of agriculture, food and wine, The University of Adelaide, Australia. p 177

  • Anugrahwati DR, Shepherd KW, Verlin DC, Zhang P, Mirzaghaderi G, Walker E, Francki MG, Dundas IS (2008) Isolation of wheat-rye 1RS recombinants that break the linkage between stem rust resistance gene SrR and secalin. Genome 51:341–349

    Article  PubMed  CAS  Google Scholar 

  • Bariana HS, Brown GN, Bansal UK, Miah H, Standen GE, Lu M (2007) Breeding triple rust resistant wheat cultivars for Australia using conventional and marker-assisted selection technologies. Aust J Agric Res 58:576–587

    Article  Google Scholar 

  • Barloy D, Lemoine J, Abelard P, Tanguy AM, Rivoal R, Jahier J (2007) Marker-assisted pyramiding of two cereal cyst nematode resistance genes from Aegilops variabilis in wheat. Mol Breed 20:31–40

    Article  CAS  Google Scholar 

  • Dundas IS, Shepherd KW (1998) Shortening the Agropyron chromosome segment carrying gene Sr26 utilizing chromosome engineering and molecular markers. In: Slinkard AE (Ed) Proceedings 9th international wheat genetics symposium University Extension Press, University of Saskatoon, pp 35–37

  • Dundas IS, Verlin DC, Park RF, Bariana HS, Anugrahwati DR, Shepherd KW, McIntosh RA, Islam AKMR (2004) Progress in development of new rust resistant wheat using chromosomes from uncultivated relatives. In: Black CK, Panozzo JF, Rebetzke GJ (eds) Proc 54th Australian Cereal Chem Conf 11th Wheat Breed Assembly. Canberra, Australia, pp 122–124

    Google Scholar 

  • Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87

    Article  Google Scholar 

  • Graybosch RA, Peterson CJ, Hansen LE, Worral D, Shelton DR, Lukaszewski AJ (1993) Comparative flour quality and protein characteristics of 1BL/1RS and 1AL/1RS wheat-rye translocations. J Cereal Sci 17:95–106

    Article  CAS  Google Scholar 

  • Jin Y, Singh RP, Ward RW, Wanyera R, Kinyua M, Njau P, Fetch T, Pretorius ZA, Yahyaoui A (2007) Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 91:1096–1099

    Article  Google Scholar 

  • Jin Y, Szabo LJ, Pretorius ZA, Singh RP, Ward R, Fetch T Jr (2008) Detection of virulence to resistance gene Sr24 within race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 92:923–926

    Article  Google Scholar 

  • Kloppers FJ, Pretorious ZA (1997) Effects of combinations amongst genes Lr13, Lr34 and Lr37 on components of resistance in wheat to leaf rust. Plant Pathol 46:737–750

    Article  Google Scholar 

  • Kuchel H, Fox R, Reinheimer J, Mosionek L, Willey N, Bariana H, Jefferies S (2007) The successful application of a marker-assisted wheat breeding strategy. Mol Breed 20:295–308

    Article  Google Scholar 

  • Leonard KJ, Szabo LJ (2005) Stem rust of small grains and grasses caused by Puccinia graminis. Mol Plant Pathol 6:99–111

    Article  PubMed  Google Scholar 

  • Liu J, Liu D, Tao W, Li W, Wang S, Chen P, Cheng S, Gao D (2000) Molecular marker-facilitated pyramiding of different genes for powdery mildew resistance in wheat. Plant Breed 119:21–24

    Article  CAS  Google Scholar 

  • Liu S, Yu L-X, Singh RP, Jin Y, Sorrells ME, Anderson JA (2009) Diagnostic and co-dominant PCR markers for wheat stem rust resistance genes Sr25 and Sr26. Theor Appl Genet. doi:10.1007/s00122-009-1186-z

  • Lukaszewski AJ (2000) Manipulation of the 1RS.1BL translocation in wheat by induced homoeologous recombination. Crop Sci 40:216–225

    Article  CAS  Google Scholar 

  • Mago R, Spielmeyer W, Lawrence GJ, Lagudah ES, Ellis JG, Pryor A (2002) Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theor Appl Genet 104:1317–1324

    Article  PubMed  CAS  Google Scholar 

  • Mago R, Spielmeyer W, Lawrence GJ, Ellis JG, Pryor A (2004) Resistance genes for rye stem rust (SrR) and barley powdery mildew (Mla) are located in syntenic regions on short arm of chromosome 1. Genome 47:112–121

    Article  PubMed  CAS  Google Scholar 

  • Mago R, Bariana HS, Dundas IS, Spielmeyer W, Lawrence GJ, Pryor AJ, Ellis JG (2005) Development of PCR markers for the selection of wheat stem rust resistance genes Sr24 and Sr26 in diverse wheat germplasm. Theor Appl Genet 111:496–504

    Article  PubMed  CAS  Google Scholar 

  • Mago R, Zhang P, Bariana HS, Verlin DC, Bansal UK, Ellis JG, Dundas IS (2009) Development of wheat lines carrying stem rust resistance gene Sr39 with reduced Aegilops speltoides chromatin and simple PCR markers for marker-assisted selection. Theor Appl Genet 119:1441–1450

    Article  PubMed  CAS  Google Scholar 

  • McIntosh RA, Dyck PL, Green GJ (1976) Inheritance of leaf rust and stem rust resistances in wheat varieties Agent and Agatha. Aust J Agric Res 28:37–45

    Article  Google Scholar 

  • McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO Publications, Victoria

    Google Scholar 

  • Park RF (2007) Stem rust of wheat in Australia. Aust J Agric Res 58:558–566

    Article  Google Scholar 

  • Samsampour D, Zanjani M, Singh A, Pallavi JK, Prabhu KV (2009) Marker assisted selection to pyramid seedling resistance gene Lr24 and adult plant resistance gene Lr48 for leaf rust resistance in wheat. Ind J Genet 69:1–9

    CAS  Google Scholar 

  • Singh RP, Hodson DP, Jin Y, Huerta-Espino J, Kinyua MG, Wanyera R, Njau P, Ward RW (2006) Current status, likely migrations and strategies to mitigate the threat to wheat production from race Ug99 (TTKS) of stem rust pathogen. CAB Reviews: perspectives in agriculture, veterinary science, nutrition and natural resources, 1, No. 054, p 13

  • Sitch LA, Snape JW (1986) Doubled haploid production in winter wheat and triticale genotypes, using Hordeum bulbosum system. Euphytica 35:1045–1051

    Article  Google Scholar 

  • Stakman EC, Stewart DM, Loegering WQ (1962) Identification of physiologic races of Puccinia graminis var. tritici. United States Department of Agriculture, Agricultural Research Service, E617. United States Government Printing Office, Washington, p 53

  • The TT, Latter BDH, McIntosh RA, Ellison FW, Brennan PS, Fisher J, Hollamby GJ, Rathjen AJ, Wilson RE (1988) Grain yields of near isogenic lines with added genes for stem rust resistance. In: Miller TE, Koebner RMD (eds) Proceedings 7th international wheat genetics symposium. Bath Pres, Bath, pp 901–909

  • Wu S, Pumphrey M, Bai G (2009) Molecular mapping of stem-rust-resistance gene Sr40 in wheat. Crop Sci 49:1681–1686

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We are thankful to Xiaodi Xia for providing excellent technical assistance and Drs Harbans Bariana and Hanif Miah, PBI, Cobbitty, for the stem rust tests. We are thankful to the Grains Research and Development Corporation, Australia, for financial support through the Australian Cereal Rust Control Program.

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  1. CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia

    Rohit Mago, Gregory J. Lawrence & Jeffrey G. Ellis

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  1. Rohit Mago
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  2. Gregory J. Lawrence
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  3. Jeffrey G. Ellis
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Correspondence to Rohit Mago.

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Mago, R., Lawrence, G.J. & Ellis, J.G. The application of DNA marker and doubled-haploid technology for stacking multiple stem rust resistance genes in wheat. Mol Breeding 27, 329–335 (2011). https://doi.org/10.1007/s11032-010-9434-0

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  • DOI: https://doi.org/10.1007/s11032-010-9434-0

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