Abstract
A new mutation at the acetohydroxyacid synthase (AHAS) locus on chromosome 6D of wheat was analyzed in detail because it conferred an improved resistance to the imidazolinone group of herbicides. Sequence analysis showed that the mutation was at the Ala122 position (A122T), a position in AHAS which has not to date been identified in imidazolinone resistant wheat lines even though the position has been identified in other plants and is associated with resistance. An allele-specific assay for the mutation (in the wheat line Brookton-8) was developed and used in a genetic analysis. Two mapping populations were analysed and the doubled haploid progeny from the cross Brookton-8 × Clearfield STL proved to be most informative. The AHASAla122 mutation (A122T) was allelic to the AHASSer653 mutation (S653N) in Clearfield STL (Imi1, on chromosome 6D) and hence was assigned to the chromosome 6D locus. The analysis of the doubled haploid lines in the mapping population demonstrated the greater resistance conferred by the A122T mutation because lines from the same cross and carrying either the A122T or S653N mutations could be directly compared.
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References
Anderson JA, Matthiesen L, Hegstad J (2004) Resistance to an imidazolinone herbicide is conferred by a gene on chromosome 6DL in the wheat line cv 9804. Weed Sci 52:83–90
Bernasconi P, Woodworth AR, Rosen BA, Subramanian MV, Siehl DL (1995) A naturally occurring point mutation confers broad range tolerance to herbicides that target acetolactate synthase. J Biol Chem 270:17381–17385
Bowran D, Barclay I, Jose KF (2006) Patent application number US2006/0095992 A1
GenStat 9th Edition for Windows (2006) VSN International, United Kingdom
Gerwick BC, Subramanian MV, Loney-Gallant VI, Chandler DP (1990) Mechanism of action of the 1,2,4-triazolo[1,5-a] pyrimidiines. Pestic Sci 29:357–364
Hattori J, Rutledge R, Labbe H, Bowan D, Sunohara G, Miki B (1992) Multiple resistance to sulfonylureas and imidazolinones conferred by an acetohydroxyacid synthase gene with separate mutations for selective resistance. Mol Gen Genet 232:167–173
Jander G, Baerson SR, Hudak JA, Gonzalez KA, Gruys KJ, Last RL (2003) Ethylmethanesulfonate saturation mutagenesis in Arabidopsis to determine frequency of herbicide resistance. Plant Physiol 131:139–146
Kolkman JM, Slabaugh MB, Bruniard JM, Berry S, Bushman BS, Olungu C, Maes N, Abratti G, Zambelli A, Miller JF, Leon A, Knapp SJ (2004) Acetohydroxyacid synthase mutations conferring resistance to imidazolinone or sulfonylurea herbicides in sunflower. Theor Appl Genet 109:1147–1159
Mallory-Smith CA, Thill DC, Dial MJ, Zemetra RS (1990) Inheritance of sulfonylurea herbicide resistance in Lactuca spp. Weed Technol 4:787–790
McCourt JA, Pang SS, King-Scott J, Guddat LW, Duggleby RG (2006) Herbicide-binding sites revealed in the structure of plant acetohydroxyacid synthase. Proc Natl Acad Sci USA 103:569–573
Morris CP, Harris RJ (1991) Solid phase amplification process. Granted US patent No. 6017738
Mourad G, Pandey B, King J (1993) Isolation and genetic analysis of a triazolopyrimidine-resistant mutant of Arabidopsis. J Hered 84:91–96
Newhouse KE, Smith WA, Starrett MA, Schaefer TJ, Singh BK (1992) Tolerance to imidazolinone hebicides in wheat. Plant Physiol 100:882–886
Ott K-H, Kwagh J-G, Stockton GW, Sidorov V, Kakefuda G (1996) Rational molecular design and genetic engineering of herbicide resistant crops by structure modeling and site-directed mutagenesis of acetohydroxyacid synthase. J Mol Biol 262:359–368
Patzoldt WL, Tranel PJ, Alexander AL, Schmitzer PR (2001) A common ragweed population resistant to chloransulam-methyl. Weed Sci 49:485–490
Perez-Jones A, Mallory-Smith CA, Hansen JL, Zemetra RS (2004) Introgression of an imidazolinone-resistance gene from winter wheat (Triticum aestivum L) into jointed goatgrass (Aegilops cylindrica Host). Theor Appl Genet 114:177–186
Pozniak CJ, Birk IT, O’Donoughue S, Menard C, Hucl PJ, Singh BK (2004a) Physiological and molecular characterization of mutation-derived imidazolinone resistance in spring wheat. Crop Sci 44:1434–1443
Pozniak CJ, Birk IT, O’Donoughue LS, Menard C, Hucl PJ, Singh BK (2004b) Physiological and molecular characterization of mutation- derived imidazolinone resistance in spring wheat. Crop Sci 44:1–10
Ray TB (1984) Site of action of chlorosulfuron. Inhibition of valine and isoleucine biosynthesis in plants. Plant Physiol 75:827–831
Rogowsky PM, Shepherd KW, Langridge P (1992) Polymerase chain reaction based mapping of rye involving repeated DNA sequences. Genome 35:621–626
Shaner DL, Anderson PC, Stidham MA (1984) Imidazolinones. Potent inhibitors of acetohydroxyacid synthase. Plant Physiol 76:545–546
Takahashi S, Shigematsu S, Morita A, Nezu M, Clauss JS, Williams CS (1991) A new herbicide for cotton. Proc Brighton Corp Protection Conf—Weeds 1:57–62
Tan S, Evans RR, Dahmer ML, Singh BK, Shaner DL (2005) Imidazolinone-tolerant crops: history, current status and future. Pest Manag Sci 61:246–257
Tan S, Evans R, Singh B (2006) Herbicidal inhibitors of amino acid biosynthesis and herbicide-tolerant crops. Amino Acids 30:195–204
Tranel PJ, Wright TR (2002) Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci 50:700–712
Vencill WK (ed) (2002) Herbicide handbook. Weed Society of America, Lawrence, Kansas
Zhao C, Ascenzi R, Singh BK (2005) Methods and compositions for analyzing AHASL genes. Patent No WO2005093093, BASF Aktiengesellschaft
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This project was funded within the Molecular Plant Breeding cooperative research centre (MPBCRC). The authors are grateful to David Bowran for valuable discussions in the early stages of the study and Keith Alcock for critical reading of the manuscript.
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Li, D., Barclay, I., Jose, K. et al. A mutation at the Ala122 position of acetohydroxyacid synthase (AHAS) located on chromosome 6D of wheat: improved resistance to imidazolinone and a faster assay for marker assisted selection. Mol Breeding 22, 217–225 (2008). https://doi.org/10.1007/s11032-008-9168-4
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DOI: https://doi.org/10.1007/s11032-008-9168-4