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Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding

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Abstract

The Pi20(t) gene was determined to confer a broad-spectrum resistance against diverse blast pathotypes (races) in China based on inoculation experiments utilizing 160 Chinese Magnaporthe oryzae (formerly Magnaporthe grisea) isolates, among which isolate 98095 can specifically differentiate the Pi20(t) gene present in cv. IR24. Two flanking and three co-segregating simple sequence repeat (SSR) markers for Pi20(t), located near the centromere region of chromosome 12, were identified using 526 extremely susceptible F2 plants derived from a cross of Asominori, an extremely susceptible cultivar, with resistant cultivar IR24. The SSR OSR32 was mapped at a distance of 0.2 cM from Pi20(t), and the SSR RM28050 was mapped to the other side of Pi20(t) at a distance of 0.4 cM. The other three SSR markers, RM1337, RM5364 and RM7102, co-segregated with Pi20(t). RM1337 and RM5364 were found to be reliable markers of resistance conditioned by Pi20(t) in a wide range of elite rice germplasm in China. As such, they are useful tags in marker-assisted rice breeding programs aimed at incorporating Pi20(t) into advanced rice breeding lines and, ultimately, at obtaining a durable and broad spectrum of resistance to M. oryaze.

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Acknowledgements

We gratefully thank Dr. Y. Fukuta at the International Rice Research Institute for providing the rice mono-genic lines, Dr. Xinghua Wei at China National Rice Research Institute for providing some of the rice leading cultivars and Mr. Zhanling Liu and Mr. Ke Shi for their help in the inoculation experiments. This work was supported by grants from the 863 Program (Grant No. 2006AA100101), National Key Technology R&D Program (Grant no: 2006BAD13B01-3) the 948 Program (Grant No. 2006-G61) of China, and National Program 301 Component II (Genomic Characterization and Genetic Improvement) of Agriculture Research Service of the United States Department of Agriculture.

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Author notes

  1. Wei Li

    Present address: Agronomy College, Guangdong Ocean University, Zhanjiang, 524088, China

  2. Dongyi Huang

    Present address: Agronomy College, Hainan University, Danzhou, 571737, China

Authors and Affiliations

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China

    Wei Li

  2. Institute of Crop Science, Chinese Academy of Agricultural Sciences and the National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, 100081, China

    Wei Li, Cailin Lei, Zhijun Cheng, Dongyi Huang, Jiulin Wang, Jiankang Wang, Xin Zhang, Ning Su, Xiuping Guo, Huqu Zhai & Jianmin Wan

  3. USDA-ARS Dale Bumpers National Rice Research Center, 2890 HWY 130 E, Stuttgart, AR, 72160, USA

    Yulin Jia

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  1. Wei Li
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Correspondence to Jianmin Wan.

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Wei Li and Cailin Lei contributed equally to this work.

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Li, W., Lei, C., Cheng, Z. et al. Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding. Mol Breeding 22, 141–149 (2008). https://doi.org/10.1007/s11032-008-9163-9

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  • DOI: https://doi.org/10.1007/s11032-008-9163-9

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