Abstract
Male sterile cv Desiree has been generated for use with other transgenic traits where hybridisation of a potato crop with wild relatives might occur. A ribosomal inactivating protein from maize (b32RIP) was expressed under the control of a tapetal specific promoter (TA29). Its known low activity against plant ribosomes was not correlated with either a lower incidence or less complete male sterility than occurred for similar lines expressing an RNAse (barnase) from Bacillus amyloliquefaciens. The b32RIP lines had a lower incidence of abnormal vegetative growth than barnase lines. Pollen grains of eight b32RIP lines and two barnase lines rarely showed uptake of the fluorophore fluorescein diacetate unlike the wild type Desiree. Pollen tubes formed very infrequently for sterile lines in conditions where this occurred for >80% of pollen of an untransformed control. Hand crossing with pollen from wild type but not the male sterile lines achieved fertilisation. Histological section of anthers for one b32RIP line confirmed its pollen grains had not matured in contrast to control plants. This is the first report of b32RIP having activity in planta against plant cells.
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References
H. Ahmad S. Galili J. Gressel (2004) ArticleTitleTandem constructs to mitigate transgene persistence: tobacco as a model Mol. Ecol. 13 697–710
H.W. Bass C. Webster G.R. O’Brian J.K.M. Roberts R.S. Boston (1992) ArticleTitleA maize ribosome-inactivating protein is controlled by the transcriptional activator Opaque-2 The Plant Cell 4 225–234 Occurrence Handle1633495 Occurrence Handle1:CAS:528:DyaK38XitlWqtb4%3D
H.W. Bass G.R. O’Brian R.S. Boston (1995) ArticleTitleCloning and sequencing of a second ribosome-inactivating protein gene from maize Plant Physiol. 107 661–662 Occurrence Handle10.1104/pp.107.2.661 Occurrence Handle7724682 Occurrence Handle1:CAS:528:DyaK2MXjvVGgsbc%3D
R.A. Boydston M.D. Seymour (2002) ArticleTitleVolunteer potato (Solanum tuberosum) control with herbicides and cultivation of onion (Allium cepa) Weed Technol. 16 620–626 Occurrence Handle1:CAS:528:DC%2BD38XnvVSqsbw%3D
L. Burbank (1921) How Plants are Trained to Work for Man: Grafting and Budding Collier New York 352
D.G. Burgess E.J. Ralston W.G. Hanson M. Heckert M. Ho T. Jenq J.M. Palys K. Tang N. Gutterson (2002) ArticleTitleA novel, two-component system for cell lethality and its use in engineering nuclear male-sterility in plants Plant J. 31 113–125 Occurrence Handle10.1046/j.1365-313X.2002.01330.x Occurrence Handle12100487 Occurrence Handle1:CAS:528:DC%2BD38XmsVWrs7g%3D
C. Celis M. Scurrah S.E. Cowgill S. Chumbiauca J. Green J. Franco G. Main D.T. Keizenbrink R.G.F. Visser H.J. Atkinson (2004) ArticleTitleEnvironmental biosafety and transgenic potato in a centre of this crops diversity Nature 432 222–225 Occurrence Handle10.1038/nature03048 Occurrence Handle15538370 Occurrence Handle1:CAS:528:DC%2BD2cXpsF2gtrk%3D
H.-J. Cho S. Kim M. Kim B-D. Kim (2001) ArticleTitleProduction of transgenic male sterile tobacco plants with the cDNA encoding a ribosome inactivating protein in Dianthus sinensis L Mol. Cells 11 326–333 Occurrence Handle11459222 Occurrence Handle1:CAS:528:DC%2BD3MXltVKjsrg%3D
M.J. Chrispeels D.E. Sadava (1994) Plants Genes and Agriculture Jones and Bartlett Publishers International London, England 400–433
A.J. Conner P.J. Dale (1996) ArticleTitleReconsideration of pollen dispersal data from field trials of transgenic potato Theor. Appl. Genet. 92 505–508
K. Davies F. Milne A. Golden (1999) Potato Volunteers and their Management Technical Notes (T480) ISSN 0142 7695 Scottish Agricultural College West Mains RoadEdinburghUK
M. De Block D. Debrouwer T. Moens (1997) ArticleTitleThe development of nuclear male sterility in wheat. Expression of the barnase gene under the control of tapetum specific promoters Theor. Appl. Genet. 95 125–131 Occurrence Handle1:CAS:528:DyaK2sXlvVaktLY%3D
Dietze J., Blau A. and Willmitzer L. 1995. Agrobacterium-mediated transformation of potato (Solanum tuberosum). In: Potrykus I. and Spangenberg G. (Eds).Gene Transfer to Plants. Springer Lab manual, pp.24–30.
R. Eijlander W.J. Stiekma (1994) ArticleTitleBiological containment of potato (Solanum tuberosum): outcrossing to the related wild species black nightshade (Solanum nigrum) and bittersweet (Solanum dulcamara) Sex. Plant Reprod. 7 29–40 Occurrence Handle10.1007/BF00241885
N.C. Ellstrand H.C. Prentice J.F. Hancock (1999) ArticleTitleGene flow and introgression from domesticated plants into their wild relatives Annu. Rev. Ecol. Syst. 30 539–563 Occurrence Handle10.1146/annurev.ecolsys.30.1.539
J. Gressel (1999) ArticleTitleTandem constructs: preventing the rise of superweeds TIBTECH. 17 361–366 Occurrence Handle1:CAS:528:DyaK1MXmtVKmsbw%3D
M.D. Halfhill B. Zhu S.I. Warwick P.L. Millwood R.J. Raymer A.K. Weissinger Jr. C.N. Stewart (2004) ArticleTitleHybridisation and backcrossing between transgenic oilseed rape and two related weed species under field conditions Environ. Biosafety Res. 3 73–81 Occurrence Handle10.1051/ebr:2004007 Occurrence Handle15612504
J.F. Hancock (2003) ArticleTitleA framework for assessing the risk of transgenic crops BioScience. 53 512–519
R.W. Hartley (1988) ArticleTitleBarnase and barstar-expression of its cloned inhibitors permits expression of a cloned ribonuclease J. Mol. Biol. 202 913–915 Occurrence Handle10.1016/0022-2836(88)90568-2 Occurrence Handle3050134 Occurrence Handle1:CAS:528:DyaL1MXhtV2it74%3D
J. Heslop-Harrison Y. Heslop-Harrison (1970) ArticleTitleEvaluation of pollen viability by enzymatically induced fluorescein diacetate Stain Tech. 45 115–120 Occurrence Handle1:CAS:528:DyaE3cXkt1ajtrg%3D
T.D. Hey M. Hartley T.A. Walsh (1995) ArticleTitleMaize ribosome-inactivating protein (b-32) Plant Phsyiol. 107 1323–1332 Occurrence Handle1:CAS:528:DyaK2MXltVClsLk%3D
R.J. Hijmans D.M. Spooner (2001) ArticleTitleGeographic distribution of wild potato species Am. J. Bot. 88 2101–2112
A. Jagannath P. Bandyopadhyay N. Arumugam V. Gupta P. Kumar D. Pental (2001) ArticleTitleThe use of spacer DNA fragment insulates the tissue-specific expression of a cytotoxic gene (barnase) and allows high-frequency generation of transgenic male sterile lines in Brassica juncea L Mol. Breeding 8 11–23 Occurrence Handle10.1023/A:1011916216191 Occurrence Handle1:CAS:528:DC%2BD3MXotl2ks7s%3D
A. Jagannath N. Arumugam V. Gupta A. Pradhan P. Kumar Burma D. Pental (2002) ArticleTitleDevelopment of transgenic barstar lines and identification of a male sterile (barnase)/restorer (barstar) combination for heterosis breeding in Indian oilseed mustard (Brassica juncea) Curr. Sci. 82 IssueID(1 46–52 Occurrence Handle1:CAS:528:DC%2BD38XhsVWgtLw%3D
T. Komari Y. Hiei Y. Saito N. Murai T. Kumashiro (1996) ArticleTitleVectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers Plant J. 10 165–174 Occurrence Handle10.1046/j.1365-313X.1996.10010165.x Occurrence Handle8758986 Occurrence Handle1:CAS:528:DyaK28XltVamtrk%3D
J.E. Krawetz R.S. Boston (2000) ArticleTitleSubstrate specificity of a maize ribosomal-inactivating protein differs across taxa Eur. J. Biochem. 267 1966–1974 Occurrence Handle10.1046/j.1432-1327.2000.01200.x Occurrence Handle10727935 Occurrence Handle1:CAS:528:DC%2BD3cXisVCisLY%3D
S. Krishnakumar D.G. Oppenheimer (1999) ArticleTitleExtragenic suppressors of the Arabidopsis zwi-3 mutation identify new genes that function in trichome branch formation and pollen tube growth Development 126 3079–3088 Occurrence Handle10375500 Occurrence Handle1:CAS:528:DyaK1MXlt1eqs7g%3D
RM. Lawson (1983) ArticleTitleTrue potato seeds as arable weeds Potato Res. 26 237–246
J. Lemmetyinen K. Keinonen T. Sopanen (2004) ArticleTitlePrevention of the flowering of a treeSilver Birch Mol. Breeding 13 243–249 Occurrence Handle10.1023/B:MOLB.0000022525.96200.53 Occurrence Handle1:CAS:528:DC%2BD2cXisl2msL8%3D
J.K. Lodge W.K. Kaniewski N.E. tumer (1993) ArticleTitleBroad spectrum virus resistance in transgenic plants expressing pokeweed anti-viral protein Proc. Natl. Acad. Sci. USA 90 7089–7093 Occurrence Handle8346221 Occurrence Handle1:CAS:528:DyaK3sXltlGktbs%3D
P.J.W. Lutman (1977) ArticleTitleInvestigations into some aspects of the biology of potatoes as weeds Weed Res. 17 123–132
C. Mariani M. De Beuckeleer J. Truettner J. Leemans R.B. Goldeberg (1990) ArticleTitleInduction of male sterility in plants by chimeric ribonuclease gene Nature 347 737–741 Occurrence Handle10.1038/347737a0 Occurrence Handle1:CAS:528:DyaK3MXhvV2jsQ%3D%3D
TD. McKnight MT. Lillis R.B. Simpson (1987) ArticleTitleSegregation of genes transferred to one plant cell from two separate Agrobacterium strains Plant Mol. Biol. 8 439–445 Occurrence Handle10.1007/BF00017989 Occurrence Handle1:CAS:528:DyaL2sXktlCisLc%3D
M. Miller L. Tagliani N. Wang B. Berka D. Bidney Z.-Y. Zhao (2002) ArticleTitleHigh efficiency transgene segregation in co-transformed maize plants using an Agrobacterium tumefaciens 2 T-DNA binary system Transgenic Res. 11 381–396 Occurrence Handle10.1023/A:1016390621482 Occurrence Handle12212841 Occurrence Handle1:CAS:528:DC%2BD38Xlt1yktLs%3D
T. Murashige F. Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant 15 473–497 Occurrence Handle1:CAS:528:DyaF3sXksFKm
K. Nielsen R.S. Boston (2001) ArticleTitleRibosomal-inactivating proteins: a plant perspective Annu. Rev. Plant Physiol. Plant Mol. Biol. 52 785–816 Occurrence Handle10.1146/annurev.arplant.52.1.785 Occurrence Handle11337416 Occurrence Handle1:CAS:528:DC%2BD3MXkslWgtro%3D
W. Orczyk J. Przetakiewicz A. Nadolska-Orczyk (2003) ArticleTitleSomatic hybrids of Solanum tuberosum application to genetics and breeding Plant Cell Tissue and Organ Culture 74 1–13 Occurrence Handle10.1023/A:1023396405655 Occurrence Handle1:CAS:528:DC%2BD3sXjtVOqs70%3D
M.A. Rieger M. Lamond C. Preston S.B. Powles R.T. Roush (2002) ArticleTitlePollen-mediated movement of herbicide resistance between commercial canola fields Science 296 2386–2388 Occurrence Handle10.1126/science.1071682 Occurrence Handle12089441 Occurrence Handle1:CAS:528:DC%2BD38XltFWlu7w%3D
J. Sambrook E. Fritsch T. Maniatis (1989) Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory Press Cold Spring HarborNY
W. Shen B.G. Forde (1989) ArticleTitleEfficient transformation of Agrobacterium spp. by high voltage electroporation Nucleic Acids Res. 17 83–85
C.N. Stewart J.N. All P.L. Raymer S. Ramachandran (1997) ArticleTitleIncreased fitness of transgenic insecticidal rapeseed under insect selection pressure Mol. Ecol. 6 773–779 Occurrence Handle10.1046/j.1365-294X.1997.00239.x
C.N. Stewart M.D. Halfhill S.I. Warwick (2003) ArticleTitleTransgene introgression from genetically modified crops to their wild relatives Nat. Rev. Genet. 4 806–817 Occurrence Handle10.1038/nrg1179 Occurrence Handle14526376 Occurrence Handle1:CAS:528:DC%2BD3sXot1aksLc%3D
B.R. Trognitz (1991) ArticleTitleComparison of different pollen viability assays to evaluate pollen fertility of potato dihaploids Euphytica 56 143–148 Occurrence Handle10.1007/BF00042057
S.J. Turner C.C. Fleming (2002) ArticleTitleMultiple selection of potato cyst nematode Globodera pallida virulence on a range of potato species. I. Serial selection on Solanum-hybrids Euro. J. Plant Pathol. 108 461–467 Occurrence Handle1:CAS:528:DC%2BD38XlsFKhu7w%3D Occurrence Handle10.1023/A:1016018002152
P.E. Urwin K.M. Troth E.I. Zubko H.J. Atkinson (2001) ArticleTitleEffective transgenic resistance to Globodera pallida in potato field trials Mol. Breeding 8 95–101 Occurrence Handle10.1023/A:1011942003994 Occurrence Handle1:CAS:528:DC%2BD3MXotl2ksLw%3D
P.E. Urwin J. Green H.J. Atkinson (2003) ArticleTitleExpression of a plant cystatin confers partial resistance to Globodera; full resistance is achieved by pyramiding a cystatin with natural resistance Mol. Breeding 12 263–269 Occurrence Handle10.1023/A:1026352620308 Occurrence Handle1:CAS:528:DC%2BD3sXosVeqtbw%3D
M.M. Williams SuffixII C.V. Ransom W.M. Thompson (2004) ArticleTitleExpression of volunteer potato density on bulb onion yield and quality Weed Sci. 52 754–758 Occurrence Handle1:CAS:528:DC%2BD2cXnvFegtL0%3D
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Green, J., Fearnehough, M.T. & Atkinson, H.J. Development of Biosafe Genetically Modified Solanum tuberosum (Potato) Cultivars for Growth within a Centre of Origin of the Crop. Mol Breeding 16, 285–293 (2005). https://doi.org/10.1007/s11032-005-0997-0
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DOI: https://doi.org/10.1007/s11032-005-0997-0