Abstract
Objectives
To engineer broad spectrum resistance in potato using different expression strategies.
Results
The previously identified Ribosome-Inactivating Protein from Phytolacca heterotepala was expressed in potato under a constitutive or a wound-inducible promoter. Leaves and tubers of the plants constitutively expressing the transgene were resistant to Botrytis cinerea and Rhizoctonia solani, respectively. The wound-inducible promoter was useful in driving the expression upon wounding and fungal damage, and conferred resistance to B. cinerea. The observed differences between the expression strategies are discussed considering the benefits and features offered by the two systems.
Conclusions
Evidence is provided of the possible impact of promoter sequences to engineer BSR in plants, highlighting that the selection of a suitable expression strategy has to balance specific needs and target species.
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Supporting information
Supplementary Table 1—Primers employed and their main features.
Supplementary Table 2—Analysis of variance of the measures of the lesion area produced by B. cinerea as a function of the genotype (Desirèe, COST and IND) and time (2, 4 and 7 days following inoculations). Post-hoc test on lesions indicated that the COST and IND genotypes are not different (p = 0.125).
Supplementary Fig. 1—Statistical analysis of the severity of the symptoms 7 days following inoculation. The graph reports mean values and its standard error of the lesion area. Different letters represent statistically different groups (Tukey; p < 0.05).
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Gonzales-Salazar, R., Cecere, B., Ruocco, M. et al. A comparison between constitutive and inducible transgenic expression of the PhRIP I gene for broad-spectrum resistance against phytopathogens in potato. Biotechnol Lett 39, 1049–1058 (2017). https://doi.org/10.1007/s10529-017-2335-0
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DOI: https://doi.org/10.1007/s10529-017-2335-0