Abstract
Background and Aim: Traditionally, Celastrus paniculatus Willd. (CP) oil has been utilized as a tranquilizer and memory enhancer. The present study investigated the neuropharmacological activity and efficacy of CP oil in ameliorating scopolamine-induced cognitive impairment in rats. Experimental procedure: Cognitive deficiency was induced in rats by administration of scopolamine (2 mg/kg intraperitoneal injection) for a period of 15 days. Donepezil served as a reference drug and CP oil was tested as both preventive and curative treatments. Animals’ behaviour was assessed through the Morris water maze (MWM), novel object preference (NOR), and conditioned avoidance (CA) tests. Oxidative stress parameters, bioamine concentration (dopamine, noradrenaline, and 5-hydroxytryptamine), nerve growth factor (NGF), interleukin-6 (IL-6), nuclear factor kappa B (NF-кB), and tumor necrosis factor-alpha (TNFα) were estimated. Synaptophysin immunohistochemistry was performed. Results: Our results showed that CP oil ameliorated behavioural deficits. It reduced latency to find a hidden platform in MWM. Reduced novel object exploration time and discrimination index (p < 0.05) in the NOR. Reduced step-down latency and normalized conditioned avoidance response (p < 0.001) in the CA test. CP oil increased dopamine, serotonin, norepinephrine, superoxide dismutase (SOD), glutathione, and catalase levels. It decreased malondialdehyde (MDA), acetylcholinesterase activity, IL-6, NF-кB (P < 0.001), TNFα, and NGF levels. Treatment showed approximate typical reactivity to synaptophysin. Conclusion: Our data is suggestive that CP oil treatment improves behavioural test outcomes, increases biogenic amine concentration, and decreases acetylcholinesterase activity, and neuroinflammatory biomarkers. It also restores synaptic plasticity. It thus improves cognitive functions against scopolamine-induced amnesia in rats by improving cholinergic function.
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Data Availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Change history
24 April 2023
A Correction to this paper has been published: https://doi.org/10.1007/s11011-023-01215-5
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This research project was supported by the grant (GUJCOST/MRP/2014-15/2592) from Gujarat Council for Science and Technology (GUJCOST) received by the author Dr Jigna Samir Shah.
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KGF contributed to the background literature review, animal studies, data acquisition, and manuscript preparation. SSP contributed to data analysis, drafting, and revision of the manuscript. JSS contributed to the conception, design of the study, supervision of the work, and critical revision of the manuscript.
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The experiments were conducted as per the approved experimental protocol (IP/PCOL/MPH/17/003). The protocol was approved the Institutional Animal Ethics Committee of Nirma University, Ahmedabad, India in accordance with the guidance of Committee for Control and Supervision of Experiments on Animal (CPCSEA), Ministry of Social justice and Empowerment, Government of India.
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Faldu, K.G., Patel, S.S. & Shah, J.S. Celastrus paniculatus oil ameliorates NF-KB mediated neuroinflammation and synaptic plasticity in the scopolamine-induced cognitive impairment rat model. Metab Brain Dis 38, 1405–1419 (2023). https://doi.org/10.1007/s11011-023-01186-7
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DOI: https://doi.org/10.1007/s11011-023-01186-7