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Effects of omega-3 fatty acid supplementation during chronic maternal hypoxia on behavioral disorders in male rat offspring: the role of Trk family and oxidative stress

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Abstract

Maternal hypoxia due to a lack of blood flow and insufficient oxygen supply in the brain leads to behavioral disorders in adult offspring. Fish oil includes docosahexaenoic acid (DHA), a significant component of membrane phospholipids of nerve cells, which improved cognition, and memory. Trk family receptors are activated by hypoxic induction factor (HIF), and are involved in the neurotrophin’s protective effects at the cellular level. Here we studied the biochemical, and molecular mechanisms of the protective effect of fish oil during the chronic maternal hypoxia model on behavioral responses in male rat offspring. Pregnant female rats were randomly divided into 4 experimental groups: 1) ctr; Control rats were pregnant 2) Hyp; Pregnant female rats received hypoxia from 6 to 15th day of pregnancy, with 10% oxygen intensity, and 90% nitrogen; 3) FO; Pregnant female rats received fish oil (F8020 1 ml / day, for ten consecutive days Orally), and 4) FO / Hyp; Pregnant female rats received hypoxia plus fish oil in the same manner. Behavioral parameters were evaluated in 28-day-old male offspring. HIF-1α, TrkB, and P75 gene expression were measured in the offspring’s brain. Maternal hypoxia impaired memory performance, and locomotor activity in offspring. Besides, Trk family gene expression, and oxidative stress indicators showed a significant increase in the offspring’s brain exposed to maternal hypoxia compared to the control group. Overall, fish oil improved behavioral parameters by inhibiting oxidative stress, and the expression of Trk family receptors.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

This study was supported financially by Iran National Science Foundation, under project number 97014509. We are grateful to the Iran National Science Foundation and Research Council of the Shahid Chamran University of Ahvaz for financial support.

Funding

This work was supported by the Iran National Science Foundation, under project number 97014509. We are grateful to the Iran National Science Foundation and Research Council of the Shahid Chamran University of Ahvaz for financial support.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Zohreh Ghotbeddin, Maryam Aliheydari & Hooshyar Yaghoubi

  2. Stem Cell and Transgenic Technology Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Zohreh Ghotbeddin, Kaveh Khazaeel & Mohammad-Reza Tabandeh

  3. Department of Anatomy, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Kaveh Khazaeel

  4. Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Mohammad-Reza Tabandeh

Authors
  1. Zohreh Ghotbeddin
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  2. Kaveh Khazaeel
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  3. Mohammad-Reza Tabandeh
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  4. Maryam Aliheydari
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  5. Hooshyar Yaghoubi
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Contributions

Zohreh Ghotbeddin participated in study design, data collection, evaluation and, contributed to behavioral experiments and, was responsible for overall supervision. Kaveh Khazaeil and Mohammad Reza Tabandeh participated in study design, data collection, evaluation and contributed in other parts of the experiment. Maryam Aliheydari and Hooshyar Yaghoubi contributed to all experimental work and data statistical analysis. All the authors reviewed and approved the final manuscript.

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Correspondence to Zohreh Ghotbeddin.

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Ghotbeddin, Z., Khazaeel, K., Tabandeh, MR. et al. Effects of omega-3 fatty acid supplementation during chronic maternal hypoxia on behavioral disorders in male rat offspring: the role of Trk family and oxidative stress. Metab Brain Dis 37, 1959–1967 (2022). https://doi.org/10.1007/s11011-022-01012-6

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  • DOI: https://doi.org/10.1007/s11011-022-01012-6

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