Abstract
mTOR has been shown to be involved in the regulation of immune responses and differentiation of immune cells. This protein is a candidate molecule for unraveling the molecular mechanisms of autoimmune disorders such as multiple sclerosis (MS). We designed the current study to assess expression of MTOR, and four associated long non-coding RNAs (lncRNAs), namely SNHG1, SNHG3, SHNG5 and DANCR in the peripheral blood of patients with MS compared with healthy controls. Analysis of real-time PCR results has shown down-regulation of SNHG5 and DANCR in MS patients compared with controls. Sex of study participants had no significant effect on expression of either genes and the interaction of sex and disease on expression levels of all studied genes were insignificant. There was a significant negative correlation between expression levels of MTOR gene and disease duration. No other significant correlations were detected between genes expressions and clinical/demographic data. SNHG5 and DANCR transcript levels had AUC values of 0.88 and 0.68 in separation of patients with MS from healthy controls, respectively. Taken together, our study suggests participation of two mTOR-related lncRNAs, i.e. SNHG5 and DANCR in the pathophysiology of MS.
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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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This study was financially supported by Grant from Medical School of Shahid Beheshti University of Medical Sciences.
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SGF wrote the manuscript and revised it. MDO and MT supervised and designed the study. MG, MA, BMH and FE performed the experiment. SE analyzed the data. All authors read and approved the submitted version.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent forms were obtained from all study participants. The study protocol was approved by the ethical committee of Shahid Beheshti University of Medical Sciences. All methods were performed in accordance with the relevant guidelines and regulations.
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Akbari, M., Eshghyar, F., Gholipour, M. et al. Expression analysis of mTOR-associated lncRNAs in multiple sclerosis. Metab Brain Dis 37, 2061–2066 (2022). https://doi.org/10.1007/s11011-022-01010-8
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DOI: https://doi.org/10.1007/s11011-022-01010-8