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MicroRNA-216a inhibits neuronal apoptosis in a cellular Parkinson’s disease model by targeting Bax

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Abstract

The study found that microRNAs play an important role in Parkinson’s disease (PD). However, the function of MicroRNA-216a (miR-216a) in PD is unclear. Therefore, this experiment aimed to investigate the pathogenesis of miR-216a in PD. Using the toxicity of MPP+ to polyhexamine neurons, apoptosis of SH-SY5Y neuroblastoma cells was induced at different time by MPP+ to construct a stable acute PD cell model. The effects of DNA breakage, mitochondrial membrane potential (A ^ m), caspase-3 activity and nucleosome enrichment on cell apoptosis were detected by flow cytometry, TUNEL. MPP+ increased the toxic effects of dopaminergic neurons in a PD model. The introduction of miR-216a inhibited MPP + -induced neuronal apoptosis. The main manifestations were the decreased levels of positive rate of Tunel cells, caspase 3 activity and nucleosome enrichment factor. Bax was a direct target of miR-216a. In addition, Bax overexpression reversed the effects of miR-216a on neural cells. Bax downstream factors were also involved in miR-216a regulation of MPP + -triggered neuronal apoptosis. miR-216a regulated the progression of PD by regulating Bax, and miR-216a may be a potential target for PD.

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Authors and Affiliations

  1. Department of Psychology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, 710061, People’s Republic of China

    Xiaobo Yang

  2. Department of Neurology, The First Affiliated Hospital of Xi’an Jiaotong University, No. 277 Yanta Western Road, Xi’an, Shaanxi, 710061, People’s Republic of China

    Meng Zhang, Meng Wei, Anqi Wang, Yongning Deng & Hongmei Cao

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  1. Xiaobo Yang
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  2. Meng Zhang
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Correspondence to Hongmei Cao.

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Yang, X., Zhang, M., Wei, M. et al. MicroRNA-216a inhibits neuronal apoptosis in a cellular Parkinson’s disease model by targeting Bax. Metab Brain Dis 35, 627–635 (2020). https://doi.org/10.1007/s11011-020-00546-x

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