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Protein phosphatase-2A is activated in pig brain following cardiac arrest and resuscitation

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Abstract

Protein phosphatase-2A (PP-2A) interacts with several regulators of cell death pathways and is therefore a potential component of signaling pathways linking global cerebral ischemia to cell death. Using a novel procedure to quantify PP-2A activity, we find that cardiac arrest with resuscitation and reperfusion leads to activation of PP-2A by 1.6-fold in pig brain extract and by 3.4-fold after partial purification of PP-2A. This is the first demonstration of PP-2A activation in a clinically relevant model of transient global cerebral ischemia. These results suggest that inhibition of PP-2A activity may be neuroprotective in global cerebral ischemia.

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Acknowledgement

This work was supported in part by the Department of Anesthesiology, Weill Medical College of Cornell University and the Phaekhim-Sekekos Foundation Fund for Biomedical Research.

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Authors and Affiliations

  1. Institute for Neuronal Cell Signaling, Department of Anesthesiology, Weill Medical College of Cornell University, New York, NY, 10021, USA

    Tao T. Zhang, Jimcy Platholi, Paul M. Heerdt, Hugh C. Hemmings Jr. & H. Y. L. Tung

  2. Department of Pharmacology, Weill Medical College of Cornell University, New York, NY, 10021, USA

    Jimcy Platholi, Paul M. Heerdt & Hugh C. Hemmings Jr.

  3. Laboratory of Neurobiochemistry, Institute for Neuronal Cell Signaling, Department of Anesthesiology, Weill Medical College of Cornell University, 525 East 68th Street/LC 201/ Box 50, New York, NY, 10021, USA

    H. Y. L. Tung

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  1. Tao T. Zhang
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  2. Jimcy Platholi
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  4. Hugh C. Hemmings Jr.
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  5. H. Y. L. Tung
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Correspondence to H. Y. L. Tung.

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Zhang, T.T., Platholi, J., Heerdt, P.M. et al. Protein phosphatase-2A is activated in pig brain following cardiac arrest and resuscitation. Metab Brain Dis 23, 95–104 (2008). https://doi.org/10.1007/s11011-007-9074-1

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  • DOI: https://doi.org/10.1007/s11011-007-9074-1

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