Abstract
The purpose of this paper was to explore the role of circ_0056618 and associated mechanisms in colorectal cancer (CRC). The expression of circ_0056618, proline rich and Gla domain 4 (PRRG4) mRNA and miR-411-5p was measured by quantitative real-time PCR (qPCR).The protein levels of PRRG4 and epithelial-mesenchymal transition (EMT)-related markers were detected by western blot. Cell proliferation was assessed by cell counting kit-8, EdU, and colony formation assays. Cell migration and invasion were assessed by transwell assay. Cell apoptosis was detected by flow cytometry assay. The putative relationship between miR-411-5p and circ_0056618 or PRRG4 was verified by dual-luciferase reporter assay. The effects of circ_0056618 on tumor growth in vivo were determined by animal study. Circ_0056618 and PRRG4 was upregulated, while miR-411-5p was downregulated in CRC tumor tissues and cells. Circ_0056618 knockdown or PRRG4 knockdown inhibited CRC cell proliferation, migration/invasion, EMT, and survival. Circ_0056618 positively modulated PRRG4 expression by targeting miR-411-5p. MiR-411-5p absence or PRRG4 overexpression could rescue circ_0056618 knockdown-induced inhibition on proliferation, migration/invasion, and EMT in CRC cells. Animal assay showed circ_0056618 knockdown impeded tumor growth in vivo. Circ_0056618 promoted CRC growth and development by upregulating PRRG4 expression via competitively targeting miR-411-5p.
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The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
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WC and YL: conceptualization and methodology; YZ, CL and BS: formal analysis and data curation; BZ and WC: validation and investigation; BZ, WC and YL: writing—original draft preparation and writing—review and editing; all authors: approval of final manuscript.
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The present study was approved by the ethical review committee of North China University of Science and Technology Affiliated Hospital. Written informed consent was obtained from all enrolled patients.
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11010_2022_4525_MOESM1_ESM.tif
Supplementary file1 (TIF 44868 KB) Fig. S1 The representative images of colony formation, cell migration, invasion, and apoptosis in SW620 and HCT116 cells with si-NC, si-circ_0056618, si-circ_0056618+anti-NC, or si-circ_0056618+anti-miR-411-5p transfection.
11010_2022_4525_MOESM2_ESM.tif
Supplementary file2 (TIF 46504 KB) Fig. S2 The representative images of colony formation, cell migration, invasion, and apoptosis in SW620 and HCT116 cells with si-NC, si-circ_0056618, si-circ_0056618+pcDNA, or si-circ_0056618+pcDNA-PRRG4 transfection.
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Zhang, B., Cao, W., Liu, Y. et al. Circ_0056618 enhances PRRG4 expression by competitively binding to miR-411-5p to promote the malignant progression of colorectal cancer. Mol Cell Biochem 478, 503–516 (2023). https://doi.org/10.1007/s11010-022-04525-x
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DOI: https://doi.org/10.1007/s11010-022-04525-x