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Tannic acid attenuate AKT phosphorylation to inhibit UMUC3 bladder cancer cell proliferation

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Abstract

Urothelial bladder cancer is rapidly spreading across Western countries, and therapy has shown little-to-moderate effects on bladder cancer. Thus, focusing on curbing cancer incidence has become crucial. The aim of the present study was to investigate the anticancer effects of Tannic acid (TA) in human bladder cancer. UMUC3 bladder cancer cells were treated with different concentrations of TA (0–100 µM) and tested for cell viability, colony formation, and apoptosis. The involvement of the phosphoinositide-3 kinase (PI3K)/Akt pathway in the action of TA was examined. TA treatment significantly inhibited the viability and increased percentage of apoptotic cells, thereby decreasing antiapoptotic proteins (BCL2, MCL-1, and BCL-XL) expression, resulting in the Caspase-3 activation. TA treatment decreased stem cell markers expression such as SOX2, OCT4, and NANOG. Additionally, TA treatment significantly reduced the phosphorylation levels of Akt in bladder cancer cells. Our study demonstrates the growth inhibitory effects of TA in bladder cancer cells, and highlights its potential as an anticancer agent for bladder cancer.

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All data generated or analyzed during this study are included in this published article.

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Funding

This work was supported by China Medical University and Asia University, Taiwan (CMU107-ASIA-01).

Author information

Author notes

  1. Chi-Cheng Li and Chih-Yang Huang have contributed equally to this work.

Authors and Affiliations

  1. Division of Colorectal Surgery, Department of Surgery, Taichung Veterans General Hospital, Taichung, Taiwan

    Ming-Cheng Chen

  2. Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan

    Ming-Cheng Chen

  3. Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan

    Selvaraj Annseles Rajula, Chiung-Hung Hsu & Chih-Yang Huang

  4. Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung, Taiwan

    V. Bharath Kumar

  5. Department of Nursing, MeiHo University, Pingtung, Taiwan

    Cecilia Hsuan Day

  6. Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan

    Ray-Jade Chen

  7. Department of Hematology and Oncology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan

    Tso-Fu Wang

  8. Department of Biotechnology, Bharathiar University, Coimbatore, India

    Vijaya Padma Viswanadha

  9. School of Medicine, Tzu Chi University, 701, Section 3, Chung-Yang Road, Hualien, 97004, Taiwan

    Chi-Cheng Li

  10. Center of Stem Cell & Precision Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan

    Chi-Cheng Li

  11. Department of Biological Science and Technology, Asia University, Taichung, Taiwan

    Chih-Yang Huang

  12. Center of General Education, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology, Hualien, Taiwan

    Chih-Yang Huang

  13. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan

    Chih-Yang Huang

Authors
  1. Ming-Cheng Chen
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Contributions

Conceptualization—M-CC, C-YH. Methodology—SAS. Validation—C-YH, VBK. Formal analysis—M-CC, C-HH. Investigation—C-YH, M-CC. Data curation—CHD, R-JC. Writing—original draft preparation—VBK. Writing—review and editing—T-FW, VPV, C–CL. Visualization—M-CC, C-YH. Supervision—C-YH. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Chih-Yang Huang.

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Chen, MC., Annseles Rajula, S., Bharath Kumar, V. et al. Tannic acid attenuate AKT phosphorylation to inhibit UMUC3 bladder cancer cell proliferation. Mol Cell Biochem 477, 2863–2869 (2022). https://doi.org/10.1007/s11010-022-04454-9

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  • DOI: https://doi.org/10.1007/s11010-022-04454-9

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