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LncRNA CRNDE promotes cell proliferation, migration and invasion of ovarian cancer via miR-423-5p/FSCN1 axis

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Abstract

Ovarian cancer seriously threatens the health of women. LncRNA CRNDE is known to be upregulated in ovarian cancer. However, the mechanism by which CRNDE regulates the progress of ovarian cancer is largely unknown. MTT assay was applied to measure the cell viability. Colony formation assay was used to measure the cell proliferation. Cell migration was tested by wound healing, and Transwell assay was performed to detect cell invasion. In addition, the expression of miR-423-5p, CRNDE and FSCN1 were detected by RT-qPCR and western blotting, respectively. Meanwhile, dual-luciferase reporter assay and RIP assay were performed to explore the correlation between miR-423-5p and CRNDE (or FSCN1). CRNDE and FSCN1 were upregulated in ovarian cancer cells (SKOV3, CAOV-3, IGROV1, A2780 and C13K), while miR-423-5p was downregulated. Moreover, silencing of FSCN1/CRNDE significantly decreased proliferation, migration and invasion of ovarian cancer cells (SKOV3 and CI3K) via suppressing MMP-2 and MMP-9. In addition, CRNDE could sponge miR-423-5p, and FSCN1 was confirmed to be the direct target of miR-423-5p. Furthermore, CRNDE knockdown-induced inhibition of FSCN1 was notably reversed by miR-423-5p downregulation. Knockdown of CRNDE inhibited cell proliferation, migration and invasion of ovarian cancer via miR-423-5p/FSCN1 axis. Thus, CRNDE may serve a new target for ovarian cancer.

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Data availability

All data generated or analyzed during this study are included in this published article.

Code availability

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Abbreviations

EOC:

Epithelial ovarian carcinoma

EMT:

Epithelial-mesenchymal transition

FSCN1:

Fascin-1

FBS:

Fetal bovine serum

Mut:

Mutant type

MMP-2:

Matrix metalloproteinase-2

MMP-9:

Matrix metalloproteinase-9

WT:

Wild type

RIP:

RNA immunoprecipitation

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Authors and Affiliations

  1. Department of Pathology, the First Medical Center of PLA General Hospital, Beijing, 100853, China

    Qiong Wang & Liang Wang

  2. Institute of Oncology, the Fifth Medical Center of PLA General Hospital, Beijing, 100853, China

    Ling-Xiong Wang

  3. Birth Defects Prevention and Control Technology Research Center, Chinese PLA General Hospital, Beijing, 100853, China

    Chun-Yan Zhang

  4. The Medicine Clinical Research Center, Chinese PLA General Hospital, Beijing, 100853, China

    Nan Bai

  5. Department of Pediatrics, the Seventh Medical Center of PLA General Hospital, Beijing, 100853, China

    Chen Feng

  6. Department of Obstetrics and Gynecology, the Third Medical Center of PLA General Hospital, No. 69, Yongding Road, Haidian District, Beijing, 100039, China

    Zhuo-Mei Zhang & Zhen-Zhen Gao

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  1. Qiong Wang
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  3. Chun-Yan Zhang
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  4. Nan Bai
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Contributions

QW: concepts, design, writing- original draft preparation; LXW: experimental studies; CYZ: writing- original draft preparation; NB: data acquisition, data analysis; CF: experimental studies; ZMZ: experimental studies; LW: data acquisition, data analysis; ZZG: supervision, writing- reviewing and Editing; All the authors approved for the final version.

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Correspondence to Zhen-Zhen Gao.

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Qiong Wang and Ling-Xiong Wang are first co-authors.

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Wang, Q., Wang, LX., Zhang, CY. et al. LncRNA CRNDE promotes cell proliferation, migration and invasion of ovarian cancer via miR-423-5p/FSCN1 axis. Mol Cell Biochem 477, 1477–1488 (2022). https://doi.org/10.1007/s11010-022-04382-8

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