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Expression of cell adhesion molecule, Gicerin/CD146 during the formation of heart and in the cardiac hypertrophy

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Abstract

Gicerin/CD146 is a cell adhesion molecule which belongs to the immunoglobulin (Ig) superfamily. We have reported the existence of gicerin/CD146 in the nervous system, heart, lung and smooth muscles of blood vessels. In this study, we make a cardiac hypertrophy model rat by constricting the rat aorta (AAC, ascending aortic constriction) and examined the effect on the expression of gicerin/CD146 in the heart. We found that the expression level of gicerin/CD146 was increased by the AAC treatment. Next, stretch stimulation was applied to myocardial cell line H9c2 cells to confirm that gicerin/CD146 may participate in the cellular hypertrophy model. We also treated the cells with inhibitors of MAP pathway enzymes. In cultured myocardial cells, the expression level of gicerin/CD146 was increased by the stretch stimulation and decreased by inhibiting the MAP pathway. Based on the above findings, it is suggested that the expression of gicerin/CD146 is involved in cardiac hypertrophy, and that the MAP pathway may be involved in the expression of gicerin/CD146 RNA in the cardiomyocyte. In addition, the expression level of gicerin/CD146 RNA in neonatal rats was upregulated after birth. Therefore, it is suggested that gicerin/CD146 might participate in the increase of myocardial cell volume both in the pathway of cardiac hypertrophy and in the developmental growth of heart.

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Acknowledgements

We thank Maki Saito for technical assistance for measuring aortic blood pressure.

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Authors and Affiliations

  1. Department of Medical Biochemistry, School of Pharmacy, Iwate Medical University, Morioka, Iwate, Japan

    Mami Obara & Koji Nata

  2. Department of Pharmacology, Iwate Medical School, Morioka, Iwate, Japan

    Mami Obara, Sachiko Sato, Kumi Takahashi, Yukiko Kondo & Eichi Taira

  3. Department of Molecular and Cellular Pharmacology, School of Pharmacy, Iwate Medical University, Morioka, Iwate, Japan

    Masamichi Hirose

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  1. Mami Obara
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  2. Sachiko Sato
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  3. Kumi Takahashi
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  7. Eichi Taira
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Correspondence to Eichi Taira.

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Obara, M., Sato, S., Takahashi, K. et al. Expression of cell adhesion molecule, Gicerin/CD146 during the formation of heart and in the cardiac hypertrophy. Mol Cell Biochem 476, 2021–2028 (2021). https://doi.org/10.1007/s11010-021-04068-7

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  • DOI: https://doi.org/10.1007/s11010-021-04068-7

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