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GINS2 was regulated by lncRNA XIST/miR-23a-3p to mediate proliferation and apoptosis in A375 cells

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Abstract

Melanoma ranks second in aggressive tumors, and the occurrence of metastasis in melanoma results in a persistent drop in the survival rate of patients. Therefore, it is very necessary to find a novel therapeutic method for treating melanoma. It has been reported that lncRNA XIST could promote the tumorigenesis of melanoma. However, the mechanism by which lncRNA XIST regulates the progression of melanoma remains unclear. The proliferation of A375 cells was measured by clonal formation. Cell viability was detected by MTT assay. Flow cytometry was performed to detect cell apoptosis and cycle. The level of GINS2, miR-23a-3p, and lncRNA XIST was investigated by qRT-PCR. Protein level was detected by Western blot, and the correctness of prediction results was confirmed by Dual luciferase. In present study, GINS2 and lncRNA XIST were overexpressed in melanoma, while miR-23a-3p was downregulated. Silencing of GINS2 or overexpression of miR-23a-3p reversed cell growth and promoted apoptosis in A375 cells. Mechanically, miR-23a-3p directly targeted GINS2, and XIST regulated GINS2 level though mediated miR-23a-3p. Moreover, XIST exerted its function on cell proliferation, cell viability, and promoted the cell apoptosis of A375 cells though miR-23a-3p/GINS2 axis. LncRNA XIST significantly promoted the tumorigenesis of melanoma via sponging miR-23a-3p and indirectly targeting GINS2, which can be a potential new target for treating melanoma.

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All data generated or analyzed during this study are included in this published article.

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Funding

This work was supported by 2019 Inner Mongolia Autonomous Region Natural Science Foundation project “Experimental research on the treatment of malignant melanoma by RNA interference technology targeting GINS2 (No.2019MS08041)”.

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Authors and Affiliations

  1. Department of Dermatology, Peking University Third Hospital, Beijing, 100191, People’s Republic of China

    Yu-Qin Hao

  2. Department of Dermatology, Third Affiliated Hospital of Inner Mongolia Medical University, Baotou, 014010, People’s Republic of China

    Yu-Qin Hao

  3. Department of Dermatology, Mental Health Center of Inner Mongolia Autonomous Region, Hohhot, 010000, People’s Republic of China

    Ke-Wei Liu

  4. Department of Dermatology, Halison International Peace Hospital, Hengshui, 053000, People’s Republic of China

    Xin Zhang

  5. Department of Dermatology, People’s Hospital Affiliated to Inner Mongolia Medical University, Hohhot, 010000, People’s Republic of China

    Shu-Xia Kang

  6. Department of Hematology, The Second Affiliated Hospital of Baotou Medical College, Baotou, 014010, People’s Republic of China

    Kun Zhang

  7. Inner Mongolia Medical University, Hohhot, 010000, People’s Republic of China

    Wurihan Han & Li Li

  8. Department of Orthopedics, Peking University Third Hospital, Beijing, 100191, People’s Republic of China

    Zhe-Hai Li

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  1. Yu-Qin Hao
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  7. Li Li
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Contributions

Z-HL and Y-QH designed the work. S-XK, KZ, K-WL, and XZ analyzed the data. LL drafted the work. Z-HL, K-WL, and WH revised the manuscript. All authors approved the manuscript to be published.

Corresponding author

Correspondence to Zhe-Hai Li.

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These authors declare no competing interests in this study.

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Ethical approval was obtained from the ethics committee of the Third Affiliated Hospital of Inner Mongolia Medical University and informed written consent was obtained from all patients.

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Hao, YQ., Liu, KW., Zhang, X. et al. GINS2 was regulated by lncRNA XIST/miR-23a-3p to mediate proliferation and apoptosis in A375 cells. Mol Cell Biochem 476, 1455–1465 (2021). https://doi.org/10.1007/s11010-020-04007-y

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  • DOI: https://doi.org/10.1007/s11010-020-04007-y

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