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LINC00173 promotes the apoptosis of hypertrophic scar fibroblasts through increasing β-catenin expression

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Abstract

Previous studies have demonstrated the involvement of long intergenic nonprotein coding RNA 173 (LINC00173) in several pathological disorders. However, the function of LINC00173 in the hypertrophic scar is not well understood. This study confirmed that the two transcript variants of TSV1 and TSV2 were both upregulated in hypertrophic scar fibroblasts. The overexpression of TSV1 or TSV2 promoted the apoptosis of fibroblasts, whereas the overexpression of TSV2 inhibited the proliferation of fibroblasts. RNA-sequencing (RNA-seq), Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis, and gene set enrichment analysis (GSEA) showed that phosphatidylinositol 3-kinase (PI3K)/Akt and Mitogen-activated protein kinases (MAPK) signaling might be involved in the role of LINC00173 in hypertrophic scar pathogenesis. Furthermore, the protein expression of β-catenin was upregulated in the TSV1 or TSV2 overexpression group. Overall, the study demonstrated that LINC00173 promoted the apoptosis of fibroblasts through increasing β-catenin expression, suggesting that LINC00173 might be a new target for hypertrophic scar treatment.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (81701910); the Jiangsu Maternal and Child Health Research Project (F201608); and the Science and Technology Development Foundation of Nanjing Medical University (2017NJMUZD065).

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Authors and Affiliations

  1. Department of Plastic & Cosmetic Surgery, Women’s Hospital of Nanjing Medical University (Nanjing Maternity and Child Health Care Hospital), 123rd Tianfei Street, Mochou Road, Nanjing, 210004, China

    Qian Li, Ling Chen, Hui Yan & Jun Li

  2. Department of Caridology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China

    Xin Chen

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  1. Qian Li
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  2. Xin Chen
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  3. Ling Chen
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  4. Hui Yan
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  5. Jun Li
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Contributions

Jun Li projected the experiment. Qian Li and Xin Chen performed the cell transfection and qRT-PCR experiments. Ling Chen and Hui Yan performed the bioinformatics analysis the statistical analysis. Jun Li wrote and edited the manuscript.

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Correspondence to Jun Li.

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Li, Q., Chen, X., Chen, L. et al. LINC00173 promotes the apoptosis of hypertrophic scar fibroblasts through increasing β-catenin expression. Mol Cell Biochem 476, 1005–1014 (2021). https://doi.org/10.1007/s11010-020-03966-6

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  • DOI: https://doi.org/10.1007/s11010-020-03966-6

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