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Exosomes carrying ALDOA and ALDH3A1 from irradiated lung cancer cells enhance migration and invasion of recipients by accelerating glycolysis

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Abstract

Lung cancer has been recognized as the leading cause of cancer-related death worldwide. Despite the improvements of treatment, the distant metastasis and recurrence of lung cancer caused by therapy resistance is the biggest challenge in clinical management. Extracellular vesicles named exosomes play crucial roles in intercellular communication as signaling mediators and are involved in tumor development. In this study, we isolated exosomes from irradiated lung cancer cells and co-cultured the exosomes with other lung cancer cells. It was found that cellular growth and motility of recipient cells were facilitated. High-throughput LC–MS/MS assay of exosomal proteins and Gene Ontology enrichment analyses indicated that the metabolic enzymes ALDOA and ALDH3A1 had potential contribution in exosome-enhanced motility of recipient cells, and clinical survival analysis demonstrated the close correlations between ALDOA or ALDH3A1 expression and poor prognosis of lung cancer patients. After co-culturing with exosomes derived from irradiated cancer cells, the expressions of these metabolic enzymes were elevated and the glycolytic activity was promoted in recipient cancer cells. In conclusion, our data suggested that exosomes from irradiated lung cancer cells regulated the motility of recipient cells by accelerating glycolytic process, where exosomal ALDOA and ALDH3A1 proteins were important signaling factors.

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Acknowledgements

This work was supported by the National Key R&D Program of China (No. 2017YFC0108604) and National Nature Science Foundation of China (Grant Nos. 31770910, 31570850, 81672985 and 11775052).

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Authors and Affiliations

  1. Institute of Radiation Medicine, Fudan University, No. 2094 Xie-Tu Road, Shanghai, 20032, China

    Chen Wang, Jinping Xu, Dexiao Yuan, Yang Bai, Yan Pan, Jianghong Zhang & Chunlin Shao

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  1. Chen Wang
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  2. Jinping Xu
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  3. Dexiao Yuan
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  4. Yang Bai
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  5. Yan Pan
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  7. Chunlin Shao
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Contributions

CW, JX, DY and CS conceived and designed the experiments. CW and JX carried out the experiments and analyzed the data; DY, YB, YP and JZ assisted the experiments; CW and CS wrote the manuscript.

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Correspondence to Chunlin Shao.

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Wang, C., Xu, J., Yuan, D. et al. Exosomes carrying ALDOA and ALDH3A1 from irradiated lung cancer cells enhance migration and invasion of recipients by accelerating glycolysis. Mol Cell Biochem 469, 77–87 (2020). https://doi.org/10.1007/s11010-020-03729-3

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  • DOI: https://doi.org/10.1007/s11010-020-03729-3

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