Skip to main content

Advertisement

SpringerLink
Log in

Knockdown of differentiation antagonizing non-protein coding RNA exerts anti-tumor effect by up-regulating miR-214 in endometrial carcinoma

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Differentiation antagonizing non-protein coding RNA (DANCR) is a valuable long noncoding RNA (lncRNA) that involves in the progress of various cancers. However, the functions of DANCR in endometrial carcinoma (EC) have not been validated. In the present study, we aimed to evaluate the roles of DANCR in EC and explore the underlying mechanism. Expression patterns of DANCR in EC specimens and normal control specimens were determined using qRT-PCR. DANCR was knocked down in EC cell lines (AN3CA and HEC-1B) through transfection with small interfering RNA (siRNA) targeting DANCR (si-DANCR). Cell proliferation was examined using the cell counting kit-8 (CCK-8) assay. Cell apoptosis was measured by flow cytometry. Online software starBase was used to predict the target gene of DANCR. Luciferase reporter assay was carried out to confirm the association between DANCR and the predicted target microRNA (miRNA). DANCR expression was up-regulated in EC tissues as compared to the normal control tissues. Knockdown of DANCR in AN3CA and HEC-1B cells markedly suppressed cell proliferation and induced cell apoptosis. miR-214 was found to be a target miRNA of DANCR and its expression was significantly decreased in EC tissues. Suppression of miR-214 abolished the effects of si-DANCR on cell proliferation and apoptosis in AN3CA and HEC-1B cells. DANCR played an important role in promoting tumorigenesis of EC via sponging miR-214. DANCR might serve as a therapeutic target for the treatment of EC.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

lncRNA:

Long noncoding RNA

DANCR:

Differentiation antagonizing non-protein coding RNA

EC:

Endometrial carcinoma

siRNA:

Small interfering RNA

miRNA:

microRNA

References

  1. Felix AS, Yang HP, Bell DW, Sherman ME (2017) Epidemiology of endometrial carcinoma: etiologic importance of hormonal and metabolic influences. Adv Exp Med Biol 943:3–46

    Article  CAS  PubMed  Google Scholar 

  2. Llobet D, Pallares J, Yeramian A, Santacana M, Eritja N, Velasco A et al (2009) Molecular pathology of endometrial carcinoma: practical aspects from the diagnostic and therapeutic viewpoints. J Clin Pathol 62(9):777–785

    Article  CAS  PubMed  Google Scholar 

  3. Bokhman JV (1983) Two pathogenetic types of endometrial carcinoma. Gynecol Oncol 15(1):10–17

    Article  CAS  Google Scholar 

  4. Piulats JM, Guerra E, Gil-Martin M, Roman-Canal B, Gatius S, Sanz-Pamplona R et al (2017) Molecular approaches for classifying endometrial carcinoma. Gynecol Oncol 145(1):200–207

    Article  CAS  PubMed  Google Scholar 

  5. Palisoul M, Mutch DG (2016) The clinical management of inoperable endometrial carcinoma. Expert Rev Anticancer Ther 16(5):515–521

    Article  CAS  PubMed  Google Scholar 

  6. Ransohoff JD, Wei Y, Khavari PA (2018) The functions and unique features of long intergenic non-coding RNA. Nat Rev Mol Cell Biol 19(3):143–157

    Article  CAS  PubMed  Google Scholar 

  7. Ponting CP, Oliver PL, Reik W (2009) Evolution and functions of long noncoding RNAs. Cell 136(4):629–641

    Article  CAS  PubMed  Google Scholar 

  8. Yang G, Lu X, Yuan L (2014) LncRNA: a link between RNA and cancer. Biochim Biophys Acta 11:1097–1109

    Article  CAS  Google Scholar 

  9. Martens-Uzunova ES, Bottcher R, Croce CM, Jenster G, Visakorpi T, Calin GA (2014) Long noncoding RNA in prostate, bladder, and kidney cancer. Eur Urol 65(6):1140–1151

    Article  CAS  PubMed  Google Scholar 

  10. Sun Y, Zou X, He J, Mao Y (2017) Identification of long non-coding RNAs biomarkers associated with progression of endometrial carcinoma and patient outcomes. Oncotarget 8(32):52604–52613

    PubMed  PubMed Central  Google Scholar 

  11. Smolle MA, Bullock MD, Ling H, Pichler M, Haybaeck J (2015) Long non-coding RNAs in endometrial carcinoma. Int J Mol Sci 16(11):26463–26472

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Thin KZ, Liu X, Feng X, Raveendran S, Tu JC (2018) LncRNA-DANCR: a valuable cancer related long non-coding RNA for human cancers. Pathol Res Pract 214(6):801–805

    Article  CAS  PubMed  Google Scholar 

  13. Wang J, Zhao X, Guo Z, Ma X, Song Y, Guo Y (2017) Regulation of NEAT1/miR-214-3p on the growth, migration and invasion of endometrial carcinoma cells. Arch Gynecol Obstet 295(6):1469–1475

    Article  CAS  PubMed  Google Scholar 

  14. Yuan SX, Wang J, Yang F, Tao QF, Zhang J, Wang LL et al (2016) Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma by derepression of CTNNB1. Hepatology 63(2):499–511

    Article  CAS  PubMed  Google Scholar 

  15. Zhan Y, Chen Z, Li Y, He A, He S, Gong Y et al (2018) Long non-coding RNA DANCR promotes malignant phenotypes of bladder cancer cells by modulating the miR-149/MSI2 axis as a ceRNA. J Exp Clin Cancer Res 37(1):273

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Liang H, Zhang C, Guan H, Liu J, Cui Y (2019) LncRNA DANCR promotes cervical cancer progression by upregulating ROCK1 via sponging miR-335-5p. J Cell Physiol 234(5):7266–7278

    Article  CAS  PubMed  Google Scholar 

  17. Salmena L, Poliseno L, Tay Y, Kats L, Pandolfi PP (2011) A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language? Cell 146(3):353–358

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Cheng DL, Xiang YY, Ji LJ, Lu XJ (2015) Competing endogenous RNA interplay in cancer: mechanism, methodology, and perspectives. Tumour Biol 36(2):479–488

    Article  CAS  PubMed  Google Scholar 

  19. Wang JX, Yang Y, Li K (2018) Long noncoding RNA DANCR aggravates retinoblastoma through miR-34c and miR-613 by targeting MMP-9. J Cell Physiol 233(10):6986–6995

    Article  CAS  PubMed  Google Scholar 

  20. Wang Y, Zeng X, Wang N, Zhao W, Zhang X, Teng S et al (2018) Long noncoding RNA DANCR, working as a competitive endogenous RNA, promotes ROCK1-mediated proliferation and metastasis via decoying of miR-335-5p and miR-1972 in osteosarcoma. Mol Cancer 17(1):89

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Peng R, Men J, Ma R, Wang Q, Wang Y, Sun Y et al (2017) miR-214 down-regulates ARL2 and suppresses growth and invasion of cervical cancer cells. Biochem Biophys Res Commun 484(3):623–630

    Article  CAS  PubMed  Google Scholar 

  22. Zhao X, Lu C, Chu W, Zhang Y, Zhang B, Zeng Q et al (2016) microRNA-214 governs lung cancer growth and metastasis by targeting carboxypeptidase-D. DNA Cell Biol 35(11):715–721

    Article  CAS  PubMed  Google Scholar 

  23. Zhang Q, Zhang S (2017) miR-214 promotes radioresistance in human ovarian cancer cells by targeting PETN. Biosci Rep 37(4):BSR20170327

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Zhang J, Su B, Gong C, Xi Q, Chao T (2016) miR-214 promotes apoptosis and sensitizes breast cancer cells to doxorubicin by targeting the RFWD2-p53 cascade. Biochem Biophys Res Commun 478(1):337–342

    Article  CAS  PubMed  Google Scholar 

  25. Lu Q, Xu L, Li C, Yuan Y, Huang S, Chen H (2016) miR-214 inhibits invasion and migration via downregulating GALNT7 in esophageal squamous cell cancer. Tumour Biol 37(11):14605–14614

    Article  CAS  PubMed  Google Scholar 

  26. Xu C, He T, Li Z, Liu H, Ding B (2017) Regulation of HOXA11-AS/miR-214-3p/EZH2 axis on the growth, migration and invasion of glioma cells. Biomed Pharmacother 95:1504–1513

    Article  CAS  PubMed  Google Scholar 

  27. Liu Y, Zhou H, Ma L, Hou Y, Pan J, Sun C et al (2016) MiR-214 suppressed ovarian cancer and negatively regulated semaphorin 4D. Tumour Biol 37(6):8239–8248

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Shanxian Central Hospital, No. 1 Wenhua Road, Heze, 274300, Shandong, China

    Jingli Sun, Shaofeng Gao & Cuihua Lu

Authors
  1. Jingli Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shaofeng Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cuihua Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cuihua Lu.

Ethics declarations

Conflict of interest

All authors declare that they have no conflicts of interest.

Ethical approval

This study was approved by the Ethics Committee of Shanxian Central Hospital.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sun, J., Gao, S. & Lu, C. Knockdown of differentiation antagonizing non-protein coding RNA exerts anti-tumor effect by up-regulating miR-214 in endometrial carcinoma. Mol Cell Biochem 460, 9–15 (2019). https://doi.org/10.1007/s11010-019-03565-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11010-019-03565-0

Keywords

Search

Navigation