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Possible use of miRNAs-146a and -499 expression and their polymorphisms as diagnostic markers for rheumatoid arthritis

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Abstract

Rheumatoid arthritis (RA) is a systemic autoimmune disorder affecting the peripheral joints. Different microRNAs had been investigated in RA including miRNA-146a meanwhile, miRNA-499 there were no studies to prove its expression in RA serum samples. This study was performed to investigate expression of both miRNAs-146a and -499 and their polymorphisms in Egyptian patients with RA and to evaluate their relationship with clinico-pathological data. The present study includes 108 subjects classified into two main groups: 52 RA patients and 56 unrelated healthy controls. RA patients were subclassified according to DAS28 score into inactive (23 patients) and active (29 patients). Quantitative expression of serum miRNA-146a, miRNA-499 as well as their Genotyping rs2910164 (C/G) and rs3746444 (T/C), respectively, were done to all subjects using real-time PCR. Serum miRNA-146a and -499 were significantly over expressed in RA patients, but they were not correlated to disease activity. Serum miRNA-146a was negatively correlated with anti-nuclear antibodies (ANA). miRNA-146a (rs2910164) genotyping revealed that the GG genotype and the frequency of the G allele were significantly higher in RA patients compared to the controls. miRNA-499 (rs3746444), genotyping revealed that the CC genotype and the frequency of the C allele were significantly higher. It can be concluded that both miRNAs-146a and -499 can be used as diagnostic markers for RA patients. Both miRNA-146a (rs2710164) and miRNA-499 (rs3746444) were significantly associated with RA susceptibility. The C allele of miRNA-146a (rs2710164) can be considered to be protective. On the other hand, the C allele of miRNA-499 (rs3746444) was significantly associated with RA susceptibility.

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Authors and Affiliations

  1. Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt

    Ghada Ayeldeen, Yasser Nassar, Hanan Ahmed & Olfat Shaker

  2. Department of Rheumatology and Rehabilitation, Faculty of Medicine, Cairo University, Cairo, Egypt

    Tamer Gheita

Authors
  1. Ghada Ayeldeen
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  2. Yasser Nassar
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  3. Hanan Ahmed
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  4. Olfat Shaker
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  5. Tamer Gheita
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Correspondence to Olfat Shaker.

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All authors declare that they have no conflict of interest to disclose.

Ethical approval

The study was conducted on patients with RA. This study was approved by the Ethical Committee of Kasr Al Ainy Medical Hospital and was in accordance with the principle of Helsinki Declaration.

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A written informed consent was obtained from each participant.

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Ayeldeen, G., Nassar, Y., Ahmed, H. et al. Possible use of miRNAs-146a and -499 expression and their polymorphisms as diagnostic markers for rheumatoid arthritis. Mol Cell Biochem 449, 145–156 (2018). https://doi.org/10.1007/s11010-018-3351-7

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  • DOI: https://doi.org/10.1007/s11010-018-3351-7

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