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Interferon activates promoter of Nmi gene via interferon regulator factor-1

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Abstract

N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated. We identified and cloned the promoter of Nmi gene. Sequence analysis and luciferase assays shown that an IFN-stimulated response element (ISRE) and a GC box in the promoter were essential for the basal transcription activity of Nmi gene. We also found that interferon regulatory factor 1 (IRF-1) could activate transcription of Nmi by binding to the ISRE in the promoter. Knockdown of IRF-1 decreases IFN-induced Nmi transcription. These results revealed that IRF-1 is involved in the IFN-inducible expression of Nmi.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (31370182 and 31670151).

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Authors and Affiliations

  1. Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China

    Xiao Xu, Keli Chai, Yuhang Chen, Yongquan Lin, Suzhen Zhang, Wentao Qiao & Juan Tan

  2. Biological Experiment Center, College of Life Sciences, Nankai University, Tianjin, 300071, China

    Xin Li

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  1. Xiao Xu
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  2. Keli Chai
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Correspondence to Wentao Qiao or Juan Tan.

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Xu, X., Chai, K., Chen, Y. et al. Interferon activates promoter of Nmi gene via interferon regulator factor-1. Mol Cell Biochem 441, 165–171 (2018). https://doi.org/10.1007/s11010-017-3182-y

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  • DOI: https://doi.org/10.1007/s11010-017-3182-y

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