Abstract
INMAP was first identified as an interphase nucleus and mitotic apparatus-associated protein that plays essential roles in the formation of the spindle and cell-cycle progression. Here, we report that INMAP might be conserved from prokaryotes to humans, is a truncated version of the RNA polymerase III subunit B POLR3B, and is up-regulated in several human cancer cell lines including HeLa, Bel-7402, HepG2 and BGC-823. Deletion analysis revealed that the 209–290 amino-acid region is necessary for the punctate distribution of INMAP in the nucleus. Furthermore, over-expression of INMAP inhibited the transcriptional activities of p53 and AP-1 in a dose-dependent manner. These results suggest that INMAP may function through the p53 and AP-1 pathways, thus providing a possible link of its activity with tumourigenesis. Integrating our data and those in previous studies, it can be concluded that INMAP plays dual functional roles in the coordination of mitotic kinetics with gene expression as well as in cell-fate determination and proliferation.
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Acknowledgments
This study was supported by the National Natural Science Foundation of China (no. 30971470) to Q. J. Liang, Beijing Natural Science Foundation (no. 5122017) to Q. J. Liang, the open fund of Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education to Y. B. Zheng and the open fund of Beijing Key Laboratory of Gene Resource and Molecular Development to Y. L. Zhou. We thank Dr. Yue Wang (A-STAR, Singapore) and Dr. Jian Kuang (University of Texas M.D., Anderson Cancer Center, USA) for critical reading.
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Yunlei, Z., Zhe, C., Yan, L. et al. INMAP, a novel truncated version of POLR3B, represses AP-1 and p53 transcriptional activity. Mol Cell Biochem 374, 81–89 (2013). https://doi.org/10.1007/s11010-012-1507-4
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DOI: https://doi.org/10.1007/s11010-012-1507-4