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L-FABP T94A decreased fatty acid uptake and altered hepatic triglyceride and cholesterol accumulation in Chang liver cells stably transfected with L-FABP

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Abstract

Liver fatty acid-binding protein (L-FABP, FABP1) is a highly conserved key factor in lipid metabolism. This study was undertaken to verify whether the T94A mutation in the L-FABP gene affects fatty acid uptake and intracellular esterification into specific lipid pools. Candidate SNPs were recreated using site-directed mutagenesis and tested for physical function in stably transfected Chang liver cell lines. We found that the T94A mutant of L-FABP lowered FFA uptake but had no effect on FFA efflux. L-FABP T94A-expressing cells showed decreased triglyceride content and increased cholesterol accumulation compared to the wild-type control for cells incubated with an FFA mixture (oleate: palmitate, 2:1 ratio). In conclusion, our study provided additional indications of the functional relevance of the L-FABP T94A SNP in hepatic fatty acid and lipid metabolism in humans.

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Acknowledgments

This study was supported by the National Undergraduate Student Innovation Experimental Design Project (YA07048) and the Hunan Province Science and Technology Plan (2007FG3035).

Conflict of interest

The authors report no conflicts of interest regarding this study.

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Authors and Affiliations

  1. Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, 410078, People’s Republic of China

    Na Gao, Xia Qu & Dong-Sheng Ouyang

  2. The Third Xiangya Hospital, Changsha, Hunan, 410013, People’s Republic of China

    Jin Yan

  3. The Xiangya Hospital, Changsha, Hunan, 410013, People’s Republic of China

    Qi Huang

  4. The Xiangya Medical School, Central South University, Changsha, Hunan, 410013, People’s Republic of China

    Hao-Yong Yuan

Authors
  1. Na Gao
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  2. Xia Qu
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  3. Jin Yan
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  4. Qi Huang
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  5. Hao-Yong Yuan
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  6. Dong-Sheng Ouyang
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Correspondence to Dong-Sheng Ouyang.

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Gao, N., Qu, X., Yan, J. et al. L-FABP T94A decreased fatty acid uptake and altered hepatic triglyceride and cholesterol accumulation in Chang liver cells stably transfected with L-FABP. Mol Cell Biochem 345, 207–214 (2010). https://doi.org/10.1007/s11010-010-0574-7

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  • DOI: https://doi.org/10.1007/s11010-010-0574-7

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