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Urinary bladder membrane permeability differentially induced by membrane lipid composition

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Abstract

The permeability barrier of the urothelium (covering the mammalian urinary tract) has stimulated interest in the role of the luminal membrane in the barrier function. To know how membrane lipids may affect the permeability barrier we prepare endocytic vesicles of different lipid composition entrapping a fluorescent dye (HPTS) and its quencher (DPX) using a dietary strategy (rats fed with commercial, oleic acid- or linoleic acid-enriched diets) followed by endocytosis induction. Vesicular leakage was measured by a fluorescence requenching technique. The results showed (1) endocytosed vesicles can release their content; (2) a linoleic acid-rich diet did not change either the mechanism of leakage or the amount of released material relative to the control; and (3) a oleic acid-rich diet greatly affected the mechanism of release. Thus, the dietary fatty acids can modify the urothelial cell physiology altering the pathway of endocytosed urinary fluid.

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Acknowledgments

This work was supported by grants from SECYT-UNC and CONICET, Argentina. We are grateful to María Laura Nores for her helpful criticism during the realization of this work.

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  1. Primera Cátedra de Biología Celular, H. y E., Instituto de Biología Celular-Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, CC220, Ciudad Universitaria, CP5000, Córdoba, Argentina

    Ernesto Javier Grasso & Reyna Olga Calderón

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  1. Ernesto Javier Grasso
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  2. Reyna Olga Calderón
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Correspondence to Reyna Olga Calderón.

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Grasso, E.J., Calderón, R.O. Urinary bladder membrane permeability differentially induced by membrane lipid composition. Mol Cell Biochem 330, 163–169 (2009). https://doi.org/10.1007/s11010-009-0129-y

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  • DOI: https://doi.org/10.1007/s11010-009-0129-y

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