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Association of −394C>G and −420C>G polymorphisms in the RETN gene with T2DM and CHD and a new potential SNP might be exist in exon 3 of RETN gene in Chinese

Molecular and Cellular Biochemistry volume 330, Article number: 31 (2009) Cite this article

Abstract

Genetic variations of the human RETN gene are associated with metabolic phenotypes, including obesity, insulin sensitivity, diabetes, and coronary heart disease (CHD). There are few studies of two gene variants, −394C>G and −420C>G, in Chinese population. This study investigated the distribution of RETN gene, single nucleotide polymorphisms (SNPs), in Chinese Han population and the association of the polymorphisms with type 2 diabetes mellitus (T2DM) and CHD. In a population-based, case–control genetic association study, a total of 961 subjects were recruited from the community, including 318 T2DM patients, 273 CHD patients, and 370 unrelated healthy control individuals. Serum lipid levels were detected. Two SNPs of RETN gene, −394C>G and −420C>G, were genotyped by PCR-RFLP. Unknown Polymorphisms were screened with the technique of denaturing high performance liquid chromatography (DHPLC). The frequencies of RETN −394G allele in T2DM group, CHD group, and control group were 0.3066, 0.3555, and 0.3481, respectively, which are met with the Hardy–Weinberg equilibrium. There is a significant difference of the comparison of sex in T2DM group of RETN gene SNP-394C>G (P < 0.05). Compared with controls, there was no significant difference in the distribution of genotypes and allele frequencies of −394C>G polymorphic site in T2DM patients and CHD patients, respectively. No direct association was found between the −394C>G polymorphism and T2DM or CHD. The frequencies of RETN −420G allele in T2DM group, CHD group, and control group were 0.4009, 0.3725, and 0.3859, respectively, which are met with the Hardy–Weinberg equilibrium. The frequencies of RETN −420G allele in T2DM groups and control groups of Chinese population are significantly different from those in European population (0.40 vs. 0.27, 0.39 vs. 0.26) (P < 0.01). Compared with controls, there was no significant difference in distribution of genotypes and allele frequencies of −420C>G polymorphic site in T2DM patients and CHD patients, respectively. No direct association was found between the −420C>G polymorphism and T2DM or CHD. In addition, we found new potential SNP +593G>C in exon 3 of RETN gene using DHPLC. The RETN gene exhibits sex and ethnic differences. +593G>C of RETN gene might be a new potential SNP in exon 3 of RETN gene. Association between SNP −394C>G and −420C>G of RETN gene with T2DM and CHD in Chinese needs more exploration.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 30671155) and the Chinese High Tech Programs (863) from the Ministry of Science and Technology (No. 2002BA711A08). We gratefully acknowledge the medical genetics laboratory in Sichuan University for technical support and the endocrinal department in West China Hospital for assistance.

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Affiliations

  1. Department of Cell Biology and Genetics, and Center of Neuroscience, Fujian Medical University, 88 Jiaotong Road, Fuzhou, 350004, China

    Shuguang Chi, Cailian Lan, Hekun Liu, Xizhen Wang, Xuexiang Chen & Suyun Chen

  2. Department of Medical Genetics, West China Hospital, Sichuan University, and Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Chengdu, China

    Sizhong Zhang & Wei Zhang

  3. Department of Hematology, Union Hospital, Fujian Medical University, Fuzhou, China

    Yuanzhong Chen

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  1. Shuguang Chi
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Correspondence to Hekun Liu.

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Chi, S., Lan, C., Zhang, S. et al. Association of −394C>G and −420C>G polymorphisms in the RETN gene with T2DM and CHD and a new potential SNP might be exist in exon 3 of RETN gene in Chinese. Mol Cell Biochem 330, 31 (2009). https://doi.org/10.1007/s11010-009-0097-2

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Keywords

  • RETN gene
  • Type 2 diabetes mellitus (T2DM)
  • Coronary heart disease (CHD)
  • Single nucleotide polymorphism (SNP)
  • DHPLC