Abstract
Two K+ ATP channel blockers, 5-hydroxydecanoate (5-HD) and glyburide, are often used to study cross-talk between Na+/K+-ATPase and these channels. The aim of this work was to characterize the effects of these blockers on purified Na+/K+-ATPase as an aid to appropriate use of these drugs in studies on this cross-talk. In contrast to known dual effects (activating and inhibitory) of other fatty acids on Na+/K+-ATPase, 5-HD only inhibited the enzyme at concentrations exceeding those that block mitochondrial K+ ATP channels. 5-HD did not affect the ouabain sensitivity of Na+/K+-ATPase. Glyburide had both activating and inhibitory effects on Na+/K+-ATPase at concentrations used to block plasma membrane K+ ATP channels. The findings justify the use of 5-HD as specific mitochondrial channel blocker in studies on the relation of this channel to Na+/K+-ATPase, but question the use of glyburide as a specific blocker of plasma membrane K+ ATP channels, when the relation of this channel to Na+/K+-ATPase is being studied.
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References
Skou JC, Esmann M (1992) The Na, K-ATPase. J Bioenerg Biomembr 24:249–261
McDonough AA, Velotta JB, Schwinger RH et al (2002) The cardiac sodium pump: structure and function. Basic Res Cardiol 97:I19–I24
Liu L, Mohammadi K, Aynafshar B et al (2003) Role of caveolae in signal-transducing function of cardiac Na+/K+-ATPase. Am J Physiol Cell Physiol 284:C1550–C1556
Xie Z, Askari A (2002) Na+/K+-ATPase as a signal transducer. Eur J Biochem 269:2434–2439
Tian J, Liu J, Garlid KD et al (2003) Involvement of mitogen-activated protein kinases and reactive oxygen species in the inotropic action of ouabain on cardiac myocytes. A potential role for mitochondrial K(ATP) channels. Mol Cell Biochem 242:181–187
Garlid KD, Puddu PE, Pasdois P et al (2006) Inhibition of cardiac contractility by 5-hydroxydecanoate and tetraphenylphosphonium ion: a possible role of mitoKATP in response to inotropic stress. Am J Physiol Heart Circ Physiol 291:H152–H160
Pasdois P, Quinlan CL, Rissa A et al (2007) Ouabain protects rat hearts against ischemia-reperfusion injury via a pathway involving src kinase, mitoKATP, and ROS. Am J Physiol Heart Circ Physiol 292:H1470–H1478
Ahmed K, Thomas BS (1971) The effects of long chain fatty acids on sodium plus potassium ion-stimulated adenosine triphosphatase of rat brain. J Biol Chem 246:103–109
Jack-Hays MG, Xie Z, Wang Y et al (1996) Activation of Na+/K+-ATPase by fatty acids, acylglycerols, and related amphiphiles: structure-activity relationship. Biochim Biophys Acta 1279:43–48
Ribalet B, Mirell CJ, Johnson DG et al (1996) Sulfonylurea binding to a low-affinity site inhibits the Na/K-ATPase and the KATP channel in insulin-secreting cells. J Gen Physiol 107:231–241
Elmi A, Idahl LA, Sehlin J (2001) Modulation of beta-cell ouabain-sensitive 86Rb+ influx (Na+/K+ pump) by D-glucose, glibenclamide or diazoxie. Int J Exp Diabetes Res 1:265–274
Lee SY, Lee CO (2005) Inhibition of Na+-K+ pump and L-type Ca2+ channel by glibenclamide in guinea pig ventricular myocytes. J Pharmacol Exp Ther 312:61–68
Jorgensen PL (1988) Purification of Na+,K+-ATPase: enzyme sources, preparative problems, and preparation from mammalian kidney. Methods Enzymol 156:29–43
Alto LE, Elimban V, Lukas A et al (2000) Modification of heart sarcolemmal Na+/K+-ATPase activity during development of the calcium paradox. Mol Cell Biochem 207:87–94
Huang WH, Kakar SS, Askari A (1986) Activation of (Na+ + K+)-ATPase by long-chain fatty acids and fatty acyl coenzymes A. Biochem Int 12:521–528
Lanzetta PA, Alvarez LJ, Reinach PS et al (1979) An improved assay for nanomole amounts of inorganic phosphate. Anal Biochem 100:95–97
Liu L, Askari A (2006) Beta-subunit of cardiac Na+-K+-ATPase dictates the concentration of the functional enzyme in caveolae. Am J Physiol Cell Physiol 291:C569–C578
Swann AC (1984) Free fatty acids and (Na+,K+)-ATPase: effects on cation regulation, enzyme conformation, and interactions with ethanol. Arch Biochem Biophys 233:354–361
Bidard JN, Rossi B, Renaud JF et al (1984) A search for an ‘ouabain-like’ substance from the electric organ of electrophorus electricus which led to arachidonic acid and related fatty acids. Biochim Biophys Acta 769:245–252
Kakar SS, Huang WH, Askari A (1987) Control of cardiac sodium pump by long-chain acyl coenzymes A. J Biol Chem 262:42–45
Askari A, Xie ZJ, Wang YH et al (1991) A second messenger role for monoacyolglycerols is suggested by their activating effects on the sodium pump. Biochim Biophys Acta 1069:127–130
Huang WH, Xie Z, Kakar SS et al (1988) Control of the sodium pump by liponucleotides and unsaturated fatty acids: side-dependent effects in red cells. Prog Clin Biol Res 268B:401–407
Ashcroft FM (1988) Adenosine 5′-triphosphate-sensitive potassium channels. Annu Rev Neurosci 11:97–118
Notsu T, Tanaka I, Takano M et al (1992) Blockade of the ATP-sensitive K+ channel by 5-hydroxydecanoate in guinea pig ventricular myocytes. J Pharmacol Exp Ther 260:702–708
McCullough JR, Normandin DE, Conder ML et al (1991) Specific block of the anti-ischemic actions of cromakalim by sodium 5-hydroxydecanoate. Circ Res 69:949–958
Jaburek M, Yarov-Yarovoy V, Paucek P et al (1998) State-dependent inhibition of the mitochondrial KATP channel by glyburide and 5-hydroxydecanoate. J Biol Chem 273:13578–13582
Garlid KD, Paucek P, Yarov-Yarovoy V et al (1997) Cardioprotective effect of diazoxide and its interaction with mitochondrial ATP-sensitive K+ channels. Possible mechanism of cardioprotection. Circ Res 81:1072–1082
Grover GJ, Garlid KD (2000) ATP-sensitive potassium channels: a review of their cardioprotective pharmacology. J Mol Cell Cardiol 32:677–695
Tsuchiya K, Wang W, Giebisch G et al (1992) ATP is a coupling modulator of parallel Na,K-ATPase-K-channel in the renal proximal tubule. Proc Natl Acad Sci USA 89:6418–6422
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This work was supported by grants HL-36573 and HL-67842.
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Liu, L., Gable, M.E., Garlid, K.D. et al. Interactions of K+ ATP channel blockers with Na+/K+-ATPase. Mol Cell Biochem 306, 231–237 (2007). https://doi.org/10.1007/s11010-007-9574-7
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DOI: https://doi.org/10.1007/s11010-007-9574-7