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Differential subcellular localization and activity of kelch repeat proteins KLHDC1 and KLHDC2

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Abstract

We have previously identified and characterized human KLHDC2/HCLP-1, a kelch repeat protein that interacts with and inhibits transcription factor LZIP. In this study, we identified and characterized a paralog of KLHDC2 called KLHDC1. KLHDC1 and KLHDC2 share about 50% identity at the level of amino acid sequence and both gene loci localize to human chromosome 14q21.3. This cluster of KLHDC1 and KLHDC2 genes is highly conserved in vertebrates ranging from pufferfish to human. Both genes are expressed highly in skeletal muscle, but weakly in various other tissues. While KLHDC2 was predominantly found in the nucleus, KLHDC1 is a cytoplasmic protein. Neither KLHDC1 nor KLHDC2 binds to actin. In addition, KLHDC1 was unable to inhibit LZIP/CREB3-mediated transcriptional activation. Thus, KLHDC1 and KLHDC2 have differential localization and activity in cultured mammalian cells.

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Abbreviations

BD:

Gal4 DNA binding domain

HCA33:

hepatocellular carcinoma-associated antigen 33

HCF:

host cell factor 1

HCLP:

HCF-like protein

HUGO:

the human genome organization

KLHDC:

kelch domain containing protein

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Acknowledgments

We thank G.S. Hayward for gift of plasmid and A.C.S. Chun for critical reading of the manuscript. D.-Y. Jin is a Leukemia and Lymphoma Society Scholar. This work was supported by a grant to D.-Y. Jin from the Hong Kong Research Grants Council (Project HKU 7294/02M).

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Authors and Affiliations

  1. Department of Biochemistry, The University of Hong Kong, 3/F, Laboratory Block, Faculty of Medicine Building, 21 Sassoon Road, Hong Kong, China

    King-Tung Chin, Hai-Tao Xu, Yick-Pang Ching & Dong-Yan Jin

  2. Department of Pathology, The University of Hong Kong, 21 Sassoon Road, Hong Kong, China

    Hai-Tao Xu & Yick-Pang Ching

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  1. King-Tung Chin
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  2. Hai-Tao Xu
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  4. Dong-Yan Jin
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Correspondence to Dong-Yan Jin.

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Chin, KT., Xu, HT., Ching, YP. et al. Differential subcellular localization and activity of kelch repeat proteins KLHDC1 and KLHDC2. Mol Cell Biochem 296, 109–119 (2007). https://doi.org/10.1007/s11010-006-9304-6

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  • DOI: https://doi.org/10.1007/s11010-006-9304-6

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