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Role of free radicals on mechanism of radiation nephropathy

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Abstract

Purpose: In our study, after applying a single dose of 612 cGy irradiation, we aimed to observe the role of free radicals on tissue damage in the kidney caused by radiation by measuring NO level, Na/K-ATPase activity and TBARS amount which is an indicator of free radical damage. On the other hand we investigated whether the tissue damage can be prevented by vitamin A or not. Materials and methods: This study was performed on three groups: 1. Control group 2. The group to which irradiation was administrated 3. The group which was given radiation + vitamin A. The irradiation group of animals were given a single dose of gamma irradiation at a sublethal dose. In the group which was administrated both irradiation + vitamin A, vitamin A was given for two days prior to irradiation. The amount of NO was measured by ESR spectroscopy, Na/K-ATPase and TBARS were measured by spectrophotometry. Results and conclusions: As a result of radiation mediated tissue damage in the kidney, we observed a NO loss, a decrease in Na/K-ATPase activitiy and an increase in TBARS amount. Although the administration of vitamin A before radiation, did not have any effect on NO loss and decrease in Na/K-ATPase.

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Authors and Affiliations

  1. Department of Biology, Gazi University Faculty of Arts & Science, Ankara, Turkey

    B. Balabanli

  2. Department of Biochemistry, Gazi University Faculty of Medicine, Ankara, Turkey

    N. Türközkan

  3. Department of Radiation Oncology, Gazi University Faculty of Medicine, Ankara, Turkey

    M. Akmansu

  4. Department of Physics Engineering, Hacettepe University Faculty of Engineering, Ankara, Turkey

    M. Polat

  5. Department of Biology, Gazi University Faculty of Arts & Sciences, 06500, Teknikokullar Ankara, TURKEY

    B. Balabanli

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  1. B. Balabanli
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  2. N. Türközkan
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  3. M. Akmansu
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  4. M. Polat
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Correspondence to B. Balabanli.

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Balabanli, B., Türközkan, N., Akmansu, M. et al. Role of free radicals on mechanism of radiation nephropathy. Mol Cell Biochem 293, 183–186 (2006). https://doi.org/10.1007/s11010-006-9240-5

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  • DOI: https://doi.org/10.1007/s11010-006-9240-5

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