Abstract
The aim of this study was to determine the concentration of phospholipids (PL), plasmalogen components of choline (PC) and ethanolamine (PE) phosphoglycerides (PLPC, PLPE) and fatty acid profile of PL and triacylglycerols (TAG) in developing rat left ventricular myocardium between postnatal day (d) 2 and 100. The steepest increase of total PL (TPL) concentration occurs between d2 and d5, followed by a further slower increase between d20 and d40. Similar developmental changes were observed in PC and PE. The PLPE concentration rises by d10, whereas PLPC does not change during the whole period investigated, except for the transient decline on d5. The concentration of diphosphatidylglycerol (DPG) increases by d60; the steepest rise occurs between d20 and d40. Phosphatidylinositol (PI) concentration rises only by d5. The concentration of phosphatidylserine (PS) decreases between d5 and d10 and then it does not change. Sphingomyelin (SM) concentration is maintained till d10, it declines on d20 and does not change thereafter. The proportion of saturated fatty acids (SFA) increases by d5 in PC, PE, PS and TAG, and by d10 in DPG and PI. After d20 the SFA proportion gradually decline in all lipids. Monounsaturated FA (MUFA) proportion decreases in PC, PE, PI and PS from d2 till d10, and in the weaning period it tends to rise again. In contrast, in DPG and TAG the proportion of MUFA declines during the whole postnatal period. N-6 polyunsaturated FA (PUFA) decrease in all PL by d20 and rise again thereafter; in TAG they decline between d2 and d10 and return to the initial level by d100. N-3 PUFA increase in all PL during the suckling period and decline after weaning; in TAG they increase only by d5 and then they decline. This remodeling of myocardial PL and TAG composition during postnatal development may affect membrane properties and contribute to developmental changes in the function of membrane proteins and cell signaling.
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Girard J, Pascal F, Pégorier JP, Duée PH: Adaptation of glucose and fatty acid metabolism during perinatal period and suckling-weaning transition. Physiol Rev 72: 507–562, 1992
Li F, Wang X, Capasso JM, Gerdes AM: Rapid transition of cardiac myocytes from hyperplasia to hypertrophy during postnatal development. J Mol Cell Cardiol 28: 1737–1746, 1996
Wibo M, Bravo G, Godfraind T: Postnatal maturation of excitation-contraction coupling in rat ventricle in relation to the subcellular localization and surface density of 1,4-dihydropyridine and ryanodine receptors. Circ Res 68: 662–673, 1991
Gudmundsdottir A, Gudbjarnason S: Neonatal changes in fatty acid profile of phospholipids in rat heart muscle. Biochim Biophys Acta 752: 284–290, 1983
Rogers CG: Fatty acids and phospholipids of adult and newborn rat hearts and of cultured, beating neonatal rat myocardial cells. Lipids 9: 541–547, 1974
Folch J, Lees M, Sloan-Stanley GH: A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem 226: 497–509, 1957
Rouser G, Fleischer S, Yamamoto A: Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids 5: 494–496, 1970
Horrocks LA: The alk-1-enyl group content of mammalian myelin phosphoglycerides by quantitative two-dimensional thin-layer chromatography. J Lipid Res 9: 469–472, 1968
Tvrzicka E, Vecka M, Stankova B, Zak A: Analysis of fatty acids in plasma lipoproteins by gas chromatography-flame ionisation detection. Quantitative aspects. Anal Chim Acta 465: 337–350, 2002.
Ostadal B, Ostadalova I, Dhalla NS: Development of cardiac sensitivity to oxygen deficiency: Comparative and ontogenetic aspects. Physiol Rev 79: 635–659, 1999
Page E: Quantitative ultrastructural analysis in cardiac membrane physiology. Am J Physiol 235: C147–C158, 1978
Smith HE, Page E: Ultrastructural changes in rabbit heart mitochondria during the perinatal period. J Ultrastruct Res 55: 31–41, 1976
Hoch FL: Cardiolipins and biomembrane function. Biochim Biophys Acta 1113: 71–133, 1992
Paradies G, Fuggiero FN, Petrosillo G, Quagliariello El: Peroxidative damage to cardiac mitochondria: cytochrome oxidase and cardiolipin alterations. FEBS Lett 424: 155–158, 1998
Skarka L, Bardova K, Brauner P, Flachs P, Jarkovska D, Kopecky J, Ostadal B: Expression of mitochondrial uncoupling protein 3 and adenine nucleotide translocase 1 genes in developing rat heart: putative involvement in control of mitochondrial membrane potential. J Mol Cell Cardiol 35: 321–330, 2003
Bass A, Stejskalova M, Stieglerova A, Ostadal B, Samanek M: Ontogenetic development of energy-supplying enzymes in rat and guinea-pig heart. Physiol Res 50: 237–245, 2001
Kako KJ, Zaror-Behrens G, Peckett SD: Phosphatidic acid synthesis in the heart. Effect of age and species differences in the mitochondrial and microsomal synthesis. Can J Biochem 55: 308–314, 1977
Parkers JG, Thompson W: Phosphatidylethanolamine in liver mitochondria and endoplasmic reticulum: molecular species distribution and turnover. Can J Biochem 53: 698–705, 1975
Stanacev NZ, Stuhne-Sekalec L, Brooks KB, Davidson JB: Intermediary metabolism of phospholipids. The biosynthesis of phosphatidylglycerophosphate and phosphatidyl-glycerol in heart mitochondria. Biochim Biophys Acta 176: 650–653, 1969
Stuhne-Sekalec L, Wassenaar M, Jackowski G, Stanacev NZ: Comparison of the biosynthesis and composition of polyglycerophosphatides and phosphatidylinositols in mitochondria and microsomes isolated from neonatal and adult rat heart and liver. Membr Biochem 9: 29–45, 1990
Skerjans I: Mitochondrial import: Properties of precursor proteins. Cell Biol 68: 9–16, 1990
Berridge MJ: Inositol triphosphate and diacylglycerol: two interacting second messengers. Ann Rev Biochem 56: 159–193, 1995
Hamplova B, Novakova O, Kolar F, Tvrzicka E, Novak F: Protein kinase C activity and isoform expression during early postnatal development of rat myocardium. Cell Biochem Biophys 43: 105–118, 2005
Hack MH, Helmy FM: On the plasmalogenation of myocardial choline glycerophospholipid during maturation of various vertebrates. Comp Biochem Physiol 89B: 111–118, 1988
Lee T, Qian Ch, Snyder F: Biosynthesis of choline plasmalogens in neonatal rat myocytes. Arch Biochem Biophys 286: 498–503, 1991
Diagne A, Fauvel J, Record M, Chap H, Douste-Blazy L: Studies on ether phospholipids II. Comparative composition of various tissues from human, rat and guinea pig. Biochim Biophys Acta 793: 221–231, 1984
Van der Vusse GJ, Glatz JFC, Stam HCG, Reneman RS: Fatty acid homeostasis in the normoxic and ischemic heart. Physiol Rev 72: 881–940, 1992
Ford DA, Gross RW: Activation of myocardial protein kinase C by plasmalogenic diglycerides. Am J Physiol 258: C30–C36, 1990
Arthur G, Mock T, Zaborniac Ch, Choy PC: The distribution and acyl composition of plasmalogens in guinea pig heart. Lipids 20: 693–698, 1985
Gross RW: High plasmalogen and arachidonic acid content of canine myocardial sarcolemma: a fast atom bombardment mass spectroscopic and gas chromatography-mass spectroscopic characterisation. Biochemistry 23: 158–165, 1984
Huang YS, Wainwright PE, Rodden PR, Mills DE, Bulman-Fleming B, Horrobin DF: Effect of maternal dietary fats with variable n-3/n-6 ratios on tissue fatty acid composition in suckling mice. Lipids 27: 104–110, 1992
Berger A, Gershwin ME, German JB: Effects of various dietary fats on cardiolipin acyl composition during ontogeny of mice. Lipids 27: 605–612, 1992
Ghosal J, Whitworth T, Coniglio JG: Biosynthesis of fatty acids from [1-14C] acetate in the perfused rat heart. Biochim Biophys Acta 187: 576–578, 1969
Brenner RR: The desaturation step in the animal biosynthesis of polyunsaturated fatty acids. Lipids 6: 567–575, 1971
Cunnane SC, Chen ZY: Triacylglycerol: an important pool of essential fatty acids during early postnatal development in rats. Am J Physiol 262: R8–R13, 1992
Cunnane SC, Chen ZY: Quantitative changes in long-chain fatty acids during fetal and early postnatal development in rats. Am J Physiol 262: R14–R19, 1992
Chen ZY, Cunnane SC: Early postnatal development is characterized by accumulation of highly unsaturated triacylglycerols. Pediatr Res 31: 47–51, 1992
Schroedl NA, Hartzell ChR: Preferential distribution of non-esterified fatty acids to phosphatidylcholine in the neonatal mammalian myocardium. Biochem J 224: 651–659, 1984
Apstein CS, Gmeiner R, Brachfeld N: Effect of palmitate on hypoxic myocardial metabolism and contractility. Recent Adv Stud Cardiac Struct Metab 1: 136–146, 1972
Vasdev CS, Kako KJ: Incorporation of fatty acids into rat heart lipids. In vivo and in vitro studies. J Mol Cell Cardiol 9: 617–631, 1977
Gloster J, Achillea M, Harris P: Subcellular distribution of [1-14C]palmitate and [1-14C]oleate incorporated into lipids in the perfused rat heart: a comparison under isothermal and hypothermic conditions. J Mol Cell Cardiol 10: 439–448, 1978
Ghebremeskel K, Bitsanis D, Koukkou E, Lovy C, Poston L, Crawford MA: Post-natal modulation of heart and liver phosphoglyceride fatty acids in pups. Ann Nutr Metab 43: 365–373, 1999
Gudbjarnason S: Dynamics of n-3 and n-6 fatty acids in phospholipids of heart muscle. J Int Med 225: Supl 1, 117–128, 1989
Decrock F, Groscolas R, Speake BK: FA composition of heart and skeletal muscle during embryonic development of the king penguin. Lipids 37: 407–415, 2002
Cunnane SC, Armstrong JK: Long chain fatty acid composition of maternal liver lipids during pregnancy and lactation in the rat comparison of triglyceride to phospholipid. J Nutr 120: 338–345, 1990
Chen ZY, Cunnane SC: Short-term energy deficit causes net accumulation of linoleoyl-enriched triacylglycerols in rat liver. FEBS Lett 280: 393–396, 1991
Guesnet P, Alasnier C, Alessandri JM, Durand G: Modifying the n-3 fatty acid content of the maternal diet to determine the requirements of the fetal and suckling rat. Lipids 32: 527–534, 1997
Gibson RA, Kneebone GM: Fatty acid composition of human colostrum and mature breast milk. Am J Clin Nutr 34: 252–257, 1981
Vigouroux E: Dynamic study of post-natal thyroid function in the rat. Acta Endocrinol (Copenh) 83: 752–762, 1976
Hamplova B, Novakova O, Tvrzicka E, Pelouch V, Novak F: Effect of hypo- and hyperthyroid states on phospholipid composition in developing rat heart. Mol Cell Biochem 252: 295–303, 2003
Novotny J, Bourova L, Malkova O, Svoboda P, Kolar F: G proteins, beta-adrenoreceptors and beta-adrenergic responsiveness in immature and adult rat ventricular myocardium: influence of neonatal hypo- and hyperthyroidism. J Mol Cell Cardiol 31: 761–772, 1999
Emilsson A, Gudbjarnason S: Reversible alterations in fatty acid profile of glycerophospholipids in rat heart muscle induced by repeated norepinephrine administration. Biochim Biophys Acta 750: 1–6, 1983
Novak F, Tvrzicka E, Pelouch V, Jezkova J, Smik D, Novakova O: Phospholipid composition of immature rat myocardium exposed to chronic hypoxia and the effect of normoxic recovery. Collect Czech Chem Commun 69: 674–688, 2004
Jezkova J, Novakova O, Kolar F, Tvrzicka E, Neckar J, Novak F: Chronic hypoxia alters fatty acid composition of phospholipids in right and left ventricular myocardium. Mol Cell Biochem 232: 49–56, 2002
Novakova O, Pelouch V, Mrnka L, Tvrzicka E, Novak F: Phospholipid composition in mitochondria of pressure overloaded maturating rat heart. J Mol Cell Cardiol 34: A87, 2002
Gudbjarnason S, Doell B, Oskardottier G, Hallgrimsson J: Modification of cardiac phospholipids and catecholamine stress tolerance. In: C. deDuve, O. Hayaishi (eds). Tocoferol, Oxygen and Biomembranes. Elsevier, Amsterdam, 1978, pp. 297–310
Stillwell W, Wassall SR: Docosahexaenoic acid: membrane properties of a unique fatty acid. Chem Phys Lipids 126: 1–27, 2003
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Novák, F., Tvrzická, E., Hamplová, B. et al. Postnatal development of phospholipids and their fatty acid profile in rat heart. Mol Cell Biochem 293, 23–33 (2006). https://doi.org/10.1007/s11010-006-2215-8
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DOI: https://doi.org/10.1007/s11010-006-2215-8