Abstract
Our previous study showed that ferulic acid (FA) offered good radioprotection under in vitro and in vivo conditions to DNA and enhanced the DNA repair process in the peripheral blood leucocytes of mice in vivo. This study concerns radioprotection of normal versus tumor cells. Administration of FA (50 mg/kg body weight) to mice bearing fibrosarcoma tumor, 1 h prior to/ or immediately after radiation exposure (4 Gy) showed preferential radioprotection to normal cells i.e. peripheral blood leucocytes and bone marrow cells in comparison to tumor cells. This preferential protection under in vivo conditions could be attributed to poor vasculature in the tumor or peculiar characteristics of the tumor cells either to restrict its entry inside the cells or metabolize or inactivate the drug. To resolve these ex vivo study was carried out using bone marrow and tumor cells. It was found that under ex vivo condition also only bone marrow cells were protected by FA. Thus the studies revealed that FA showed preferential protection to normal cells under both in vivo and ex vivo conditions. (Mol Cell Biochem xxx: 1–10, 2005)
Similar content being viewed by others
References
Nair CKK, Parida DK, Nomura T: Radioprotectors in radiotherapy. J Radiat Res 42: 21–37, 2001
Tannehill SP, Mehta MP: Amifostine and radiation therapy: past, present and future. Semin Oncol 23: 69–77, 1996
Bors W, Heller W, Michel C, Sarn M: Flavonoids as anioxidants: determination of radical scavenging efficiencies. Method Enzymol 186: 343–355, 1990
Uma Devi P, Ganasoundari A, Rao BSS, Srinivasan KK: In vivo radioprotection by Ocimum flavonoids: survival of mice. Radiat Res 151: 74–78, 1999
Uma Devi P, Ganasoundari A: Modulation of antioxidant enzyme by Ocimum sanctum and it role in protection against radiation injury. Ind J Exp Biol 37: 262–268, 1999
Samarth RM, Kumar A: Radioprotection of Swiss albino mice by plant extract Mentha piperita (Linn). J Radiat Res 44: 101–109, 2003
Nishimura Y, Kim HS, Ikota N, Arima H, Bom HS, Kim YH, Watanabe Y, Yukawa M, Ozawa T: Radioprotective effect of chitosan in sub-lethally X-ray irradiated mice. J Radiat Res 44: 53–58, 2003
Hosseinimehr SJ, Safiee A, Mozdarani H, Akhlagpour S: Radioprotective effects of 2-iminothiazolidine derivatives against lethal doses of gamma radiation in mice. J Radiat Res 42: 401–808, 2001
Bardhan KD: Histamine H2 receptor antagonists. In: Prespective in Duodenal Ulcer, 2nd ed., Ed. S. (1981) pp. 73–84, Kline and French, Hertfordshire
Gandhi NM, Nair CKK: Radioprotection of cellular DNA in vivo by gallic acid. Mol Cell Biochem 278: 11–117, 2005
Gandhi NM, Nair CKK: Radioprotection by Terninalia chebula: some mechanistic aspects. Mol Cell Biochem 277: 43–48, 2005
Kanski J, Aksenova M, Stoyanova A, Butterfield DA: Ferulic acid antioxidant protection against hydroxyl and peroxyl radical oxidation in synaptosomal and neuronal cell culture system in vitro: Structure activity studies. J Nutr Biochem 13: 273–281, 2002
Scott BC, Butler J, Halliwell B, Aruoma OI: Evaluation of the antioxidant action of ferulic acid and catechins. Free Radic Res Commun 19: 241–253, 1993
Kikuzaki H, Hisamoto M, Hirose K, Akiyama K, Taniguchi H: Antioxidant properties of ferulic acid and its related compounds. J Agric Food Chem 50: 2161–2168, 2003
Zhouen Z, Side Y, Weizhen L, Wenfeng W, Yizun J, Nianyun L: Mechanism of reaction nitrogen dioxide radical with hydrocinnamic acid derivatives: a pulse radiolysis study. Free Radic Res 29: 13–16, 1998
Pan JX, Wang WF, Lin WZ, Lu CY, Han ZH, Yao SD, Lin NY: Interaction of hydroxycinnamic acid derivatives with the Cl3COO radical: a pulse radiolysis study. Free Radic Res 30: 241–245, 1999
Ogiwara T, Satoh K, Kadoma Y, Murakami Y, Unten S, Atsumi T, Sakagami H, Fujisawa S: Radical scavenging activity and cytotoxicity of ferulic acid. Anticancer Res 22: 2711–2717, 2002
Tanaka T, Kojima T, Kawamori T, Suzui M, Okamoto K, Mori H: Inhibition of 4-nitroquinoline 1-oxide-induced rat tongue carcinogenesis by the naturally occurring plant phenolics caffeic acid, ellagic acid, chlorogenic acid ferulic acid. Carcinogenesis 14: 1321–1325, 1993
Asanoma K, Takahashi K, Miyabe M, Yamamoto K, Yoshimi N, Mori H, Kawazoe Y: Inhibitory effect of topical application of polymerized ferulic acid, a synthetic lignin, on tumor promotion in mouse skin two-stage tumorigenesis. Carcinogenesis 15: 2069–2071, 1994
Majid YM, Hugle S, O'Brien PJ: Metabolism of caffeic acid by isolated rat hepatocytes and subcellular fraction. Toxicol Lett 133: 141–151, 2002
Luo H, Lix Xiao F, Gu F. Study on pharmacokinetics of ferulic acid and paeoniflorin in angelic-paeonia root powder. Zhong Yao Cai 26: 189–192, 2003
Rondini L, Peyrat-Maillard MN, Marsset-Baglieri A, Berset C: Sulfated ferulic acid is the main in vivo metabolite found after short-term ingestion of free ferulic acid in rats. J Agric Food Chem 50: 3037–3041, 2002
Zhao Z, Egashira Y, Sanada H: Ferulic acid sugar esters are recovered in rat plasma and urine mainly as the sulphoglucuronide of ferulic acid. Nutr 133: 1355–1361, 2003
Maurya DK, Salvi VP, Nair CKK: Radiation protection of DNA by ferulic acid under in vitro and in vivo conditions. Mol Cell Biochem 280: 209, (2005)
Waravdekar SS, Ranadive KJ: Biological testing of sulfur isosteres of carcinogenic hydrocarbons. J Natl Cancer Inst 18: 555–559, 1957
Maurya DK, Balakrishnan S, Salvi VP, Nair CKK: Protection of cellular DNA from gamma-radiation induced damages and enhancement in DNA repair by troxerutin. Mol Cell Biochem 280: 57, (2005)
Gandhi NM, Maurya DK, Salvi V, Kapoor S, Mukherjee T, Nair CKK: Radioprotection of DNA by glycyrrhizic acid through scavenging free radicals. J Radiat Res 45: 461–468, 2004
Konca K, Lankoff A, Banasik A, Lisowska H, Kuszewski T, Gozdz S, Koza Z, Wojcik A: A cross platform public domain PC image analysis program for the comet assay. Mutat Res 534: 15–20, 2003
Belli M, Sapora O, Antonella M: Molecular targets in cellular response to ionizing radiation and implications in space radiation protection. J Radiat Res 43: S13–S19, 2002
Persidis A: Cancer Multidrug resistance. Nat Biotechnol 17: 94–95, 1999
Liscovitch M, Lavie Y: Cancer Multidrug resistance: a review of recent drug discovery research. Drug 5: 349–355, 2002
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Maurya, D.K., Nair, C.K.K. Preferential radioprotection to DNA of normal tissues by ferulic acid under ex vivo and in vivo conditions in tumor bearing mice. Mol Cell Biochem 285, 181–190 (2006). https://doi.org/10.1007/s11010-005-9079-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11010-005-9079-1