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ACBP – a PPAR and SREBP modulated housekeeping gene

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Abstract

The acyl-CoA binding protein (ACBP) is a 10 kD intracellular lipid binding protein that binds and transports acyl-CoA esters. The protein is expressed in most cell types at low levels; however, expression differs markedly between different cell types with expression being particularly high in e.g. cells with a high turnover of fatty acids. We show here that the relatively high basal promoter activity of the rat ACBP gene in fibroblasts and hepatoma cells relies on sequences between −331 to −182 and on the Sp1 and NF-Y sites at −172 and −143, respectively. The basal transcription is modulated by members of the PPAR and SREBP families. In adipocytes, PPARγ is in part responsible for the induction during adipocyte differentiation, but other transcription factors appear to play a role as well. In hepatocytes, SREBP-1c is the main regulator of ACBP in response to changes in insulin levels during fasting/refeeding. PPARα counteracts this effect by stimulating ACBP expression during fasting. In addition, PPARα mediates the induction of ACBP expression in response to peroxisome proliferators. PPARα and PPARγ do not require sequences upstream of −182 for transactivation; however, SREBP-1c requires the synergistic action of sequences in intron 1 for transactivation of the ACBP promoter.

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Author notes

  1. Torben Helledie

    Present address: Institute of Molecular and Cell Biology, Proteos, Singapore

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230, Odense M, Denmark

    Ditte Neess, Pia Kiilerich, Maria B. Sandberg, Torben Helledie, Ronni Nielsen & Susanne Mandrup Ph. D.

  2. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark

    Susanne Mandrup Ph. D.

Authors
  1. Ditte Neess
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  2. Pia Kiilerich
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  3. Maria B. Sandberg
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  4. Torben Helledie
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  5. Ronni Nielsen
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  6. Susanne Mandrup Ph. D.
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Correspondence to Susanne Mandrup Ph. D..

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Neess, D., Kiilerich, P., Sandberg, M.B. et al. ACBP – a PPAR and SREBP modulated housekeeping gene. Mol Cell Biochem 284, 149–157 (2006). https://doi.org/10.1007/s11010-005-9039-9

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  • DOI: https://doi.org/10.1007/s11010-005-9039-9

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