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The function of acyl-CoA-binding protein (ACBP)/Diazepam binding inhibitor (DBI)

  • Cellular Fatty Acid-Binding Proteins II
  • Published:
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Abstract

Acyl-CoA-binding protein has been isolated independently by five different groups based on its ability to (1) displace diazepam from the GABAA receptor, (2) affect cell growth, (3) induce medium-chain acyl-CoA-ester synthesis, (4) stimulate steroid hormone synthesis, and (5) affect glucose-induced insulin secretion. In this survey evidence is presented to show that ACBP is able to act as an intracellular acyl-CoA transporter and acyl-CoA pool former. The rat ACBP genomic gene consists of 4 exons and is actively expressed in all tissues tested with highest concentration being found in liver. ACBP consists of 86 amino acid residues and contains 4 α-helices which are folded into a boomerang type of structure with α-helices 1, 2 and 4 in the one arm and α-helix 3 and an open loop in the other arm of the boomerang. ACBP is able to stimulate mitochondrial acyl-CoA synthetase by removing acyl-CoA esters from the enzyme. ACBP is also able to desorb acyl-CoA esters from immobilized membranes and transport and deliver these for mitochondrial β-oxidation. ACBP efficiently protects acetyl-CoA carboxylase and the mitochondrial ADP/ATP translocase against acyl-CoA inhibition. Finally, ACBP is shown to be able to act as an intracellular acyl-CoA pool former by overexpression in yeast. The possible role of ACBP in lipid metabolism is discussed.

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Authors and Affiliations

  1. Institute of Biochemistry, Odense University, Campusvej 55, DK-5230, Odense, Denmark

    Jens Knudsen, Susanne Mandrup, Jan Trige Rasmussen & Per Hove Andreasen

  2. Department of Chemistry, Carlsberg Laboratory, Gamle Carlsbergvej 10, DK-2500, Copenhagen Valby, Denmark

    Flemming Poulsen

  3. Department of Molecular Biology, Odense University, Campusvej 55, DK-5230, Odense M, Denmark

    Karsten Kristiansen

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  1. Jens Knudsen
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  2. Susanne Mandrup
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  3. Jan Trige Rasmussen
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  4. Per Hove Andreasen
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  5. Flemming Poulsen
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  6. Karsten Kristiansen
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Knudsen, J., Mandrup, S., Rasmussen, J.T. et al. The function of acyl-CoA-binding protein (ACBP)/Diazepam binding inhibitor (DBI). Mol Cell Biochem 123, 129–138 (1993). https://doi.org/10.1007/BF01076484

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